Flavonoid scutellarin positively regulates root length through NUTCRACKER

doi:10.1016/j.pld.2020.08.001

Plant Diversity ›› 2021, Vol. 43 ›› Issue (03): 248-254.DOI: 10.1016/j.pld.2020.08.001

• Articles • 上一篇

Flavonoid scutellarin positively regulates root length through NUTCRACKER

Xing Huang^a,b, Weiqi Li^a, Xudong Zhang^a

a Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China;
b University of the Chinese Academy of Sciences, Beijing, 100049, China

收稿日期:2020-06-25 修回日期:2020-08-03 发布日期:2021-06-28
通讯作者: Weiqi Li, Xudong Zhang
基金资助:
This research was funded by Yunnan Applied Basic Research Project (2017FB057 and 2017AB001), as well as National Natural Science Foundation of China (31700235 and 31770375). We thank Dr. Qinshi Zhao for donations of scutellarin, Dr. Yanxia Jia for assistance with confocal laser scanning microscope analysis, and Liyan Peng, Yaowen Chang, Dr. Buzhu Yu, and Dr. Peiji Zhao for assistance with the flavonoid assay, and Dr. Buzhu Yu for the chemical structure diagram.

Flavonoid scutellarin positively regulates root length through NUTCRACKER

Xing Huang^a,b, Weiqi Li^a, Xudong Zhang^a

a Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China;
b University of the Chinese Academy of Sciences, Beijing, 100049, China

Received:2020-06-25 Revised:2020-08-03 Published:2021-06-28
Contact: Weiqi Li, Xudong Zhang
Supported by:
This research was funded by Yunnan Applied Basic Research Project (2017FB057 and 2017AB001), as well as National Natural Science Foundation of China (31700235 and 31770375). We thank Dr. Qinshi Zhao for donations of scutellarin, Dr. Yanxia Jia for assistance with confocal laser scanning microscope analysis, and Liyan Peng, Yaowen Chang, Dr. Buzhu Yu, and Dr. Peiji Zhao for assistance with the flavonoid assay, and Dr. Buzhu Yu for the chemical structure diagram.

摘要/Abstract

摘要： Exploring approaches to regulate meristem is of special importance and broad interest. In this study, we found that the flavonoid scutellarin, which has a 6-hydroxyl and a 7-glucoside, increased root length through the transcription factor NUTCRACKER (NUC). This root lengthening disappeared in NUC-knockout and reappeared in NUC-rescue plants. Scutellarin induced NUC expression and promoted the division of cortex/endodermal initials. In contrast, naringenin, which has same chemical backbone but without 6-hydroxyl and with 7-hydroxyl group, showed the opposite or no effects. Our results demonstrate that scutellarin promotes root length through NUC-mediated regulatory pathways and reveal that flavonoids with and without the 6-hydroxyl and 7-glucoside have positive and negative effects on meristem size, respectively.

关键词: Root length, Meristem size, Flavonoid, Scutellarin, Naringenin, NUTCRACKER

Abstract: Exploring approaches to regulate meristem is of special importance and broad interest. In this study, we found that the flavonoid scutellarin, which has a 6-hydroxyl and a 7-glucoside, increased root length through the transcription factor NUTCRACKER (NUC). This root lengthening disappeared in NUC-knockout and reappeared in NUC-rescue plants. Scutellarin induced NUC expression and promoted the division of cortex/endodermal initials. In contrast, naringenin, which has same chemical backbone but without 6-hydroxyl and with 7-hydroxyl group, showed the opposite or no effects. Our results demonstrate that scutellarin promotes root length through NUC-mediated regulatory pathways and reveal that flavonoids with and without the 6-hydroxyl and 7-glucoside have positive and negative effects on meristem size, respectively.

Key words: Root length, Meristem size, Flavonoid, Scutellarin, Naringenin, NUTCRACKER

Xing Huang, Weiqi Li, Xudong Zhang. Flavonoid scutellarin positively regulates root length through NUTCRACKER[J]. Plant Diversity, 2021, 43(03): 248-254.

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Flavonoid scutellarin positively regulates root length through NUTCRACKER

Flavonoid scutellarin positively regulates root length through NUTCRACKER

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