Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata

doi:10.1016/j.pld.2017.07.001

Plant Diversity ›› 2017, Vol. 39 ›› Issue (04): 214-220.DOI: 10.1016/j.pld.2017.07.001

Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata

Huai Ning^a,b, Jiaojun Yu^a, Xun Gong^a

a Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China;
b University of Chinese Academy of Sciences, Beijing, 100049, China

收稿日期:2017-01-08 修回日期:2017-07-12 出版日期:2017-08-25 发布日期:2021-11-05
通讯作者: Xun Gong
作者简介:Huai Ning,E-mail addresses:ninghuai@mail.kib.ac.cn;Jiaojun Yu,E-mail addresses:yujiaojun@mail.kib.ac.cn
基金资助:
The work is supported by the National Science Foundation of China (31470336 to XG).

Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata

Huai Ning^a,b, Jiaojun Yu^a, Xun Gong^a

a Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China;
b University of Chinese Academy of Sciences, Beijing, 100049, China

Received:2017-01-08 Revised:2017-07-12 Online:2017-08-25 Published:2021-11-05
Contact: Xun Gong
Supported by:
The work is supported by the National Science Foundation of China (31470336 to XG).

摘要/Abstract

摘要： Natural hybridization has been regarded as a crucial pathway of speciation and provides the raw materials for the evolution of biodiversity. The interspecific natural hybridization of the genus Ligularia Cass. is universal and has been considered to be an important factor driving the high diversity of Ligularia species in the Hengduan Mountains, China. Although the natural hybridization between L. vellerea and L. subspicata was reported previously, the direction of hybridization was uncertain due to the limitation of sampling. Thus, in this study, we sampled more individuals and increased two fragments of chloroplast DNA on the basis of the previous study to further verify the natural hybridization between L. vellerea and L. subspicata and confirm the direction of hybridization. Based on DNA sequences (atpBrbcL, trnL-rpl32, trnQ-5'rps16, and nuclear ribosomal internal transcribed spacer region) data, we concluded that putative hybrids were primary products of hybridization between L. vellerea and L. subspicata and the hybridization was bidirectional. Moreover, sympatric L. tongolensis was not apparently involved in the hybridization. Surprisingly, some pure L. subspicata individuals showed the disaccordance between morphology and DNA data, which might indicate that introgression occurs between L. vellerea and L. subspicata.

关键词: Ligularia, Natural hybridization, Introgression, cpD

Abstract: Natural hybridization has been regarded as a crucial pathway of speciation and provides the raw materials for the evolution of biodiversity. The interspecific natural hybridization of the genus Ligularia Cass. is universal and has been considered to be an important factor driving the high diversity of Ligularia species in the Hengduan Mountains, China. Although the natural hybridization between L. vellerea and L. subspicata was reported previously, the direction of hybridization was uncertain due to the limitation of sampling. Thus, in this study, we sampled more individuals and increased two fragments of chloroplast DNA on the basis of the previous study to further verify the natural hybridization between L. vellerea and L. subspicata and confirm the direction of hybridization. Based on DNA sequences (atpBrbcL, trnL-rpl32, trnQ-5'rps16, and nuclear ribosomal internal transcribed spacer region) data, we concluded that putative hybrids were primary products of hybridization between L. vellerea and L. subspicata and the hybridization was bidirectional. Moreover, sympatric L. tongolensis was not apparently involved in the hybridization. Surprisingly, some pure L. subspicata individuals showed the disaccordance between morphology and DNA data, which might indicate that introgression occurs between L. vellerea and L. subspicata.

Key words: Ligularia, Natural hybridization, Introgression, cpD

Huai Ning, Jiaojun Yu, Xun Gong. Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata[J]. Plant Diversity, 2017, 39(04): 214-220.

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Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata

Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata

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