Plant Diversity ›› 2019, Vol. 41 ›› Issue (05): 315-322.DOI: 10.1016/j.pld.2019.07.003

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Plastome sequencing of Myripnois dioica and comparison within Asteraceae

Nan Lina,b,c, Xu Zhanga,b, Tao Dengc, Jianwen Zhangc, Aiping Menga, Hengchang Wanga, Hang Sunc, Yanxia Suna   

  1. a CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, 430074, China;
    b University of Chinese Academy of Sciences, Beijing, 100049, China;
    c CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China
  • Received:2019-03-22 Revised:2019-07-05 Online:2019-10-25 Published:2019-11-21
  • Contact: Hang Sun,E-mail;Yanxia Sun,E-mail
  • Supported by:
    This study was supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDA20050203), the National Key R&D Program of China (2017YFC0505200), the Major Program of the National Natural Science Foundation of China (31590823), and the National Natural Science Foundation of China (31570213).

Abstract: Myripnois is a monotypic shrub genus in the daisy family constricted to northern China. Although wild populations of Myripnois dioica are relatively rare, this plant may potentially be cultured as a fine ornamental. In the present study, we sequenced the complete plastome of M. dioica, generating the first plastome sequences of the subfamily Pertyoideae. The plastome of M. dioica has a typical quadripartite circular structure. A large~20-kb and a small~3-kb inversion were detected in the large single copy (LSC) region and shared by other Asteraceae species. Plastome phylogenomic analyses based on 78 Asteraceae species and three outgroups revealed four groups, corresponding to four Asteraceae subfamilies:Asteroideae, Cichorioideae, Pertyoideae and Carduoideae. Among these four subfamilies, Pertyoideae is sister to Asteroideae + Cichorioideae; Carduoideae is the most basal clade. In addition, we characterized 13 simple sequence repeats (SSRs) that may be useful in future studies on population genetics.

Key words: Myripnois, Asteraceae, Plastomes, Large inversion, Phylogenomic analyses