Plant Diversity ›› 2008, Vol. 30 ›› Issue (03): 345-350.DOI: 10.3724 SP.J.1143.2008.07224

• Articles • Previous Articles     Next Articles

Identification of Salvia Species by nrDNA ITS and cpDNA rpl16 Sequence Analyses

GUO Hui-Fang1 , KAN Xian-Zhao2 , ZHANG Ren1 , 3 , CHEN Hong-Shan1   

  1. 1 Institute of Medicinal Biotechnology , Chinese Academy of Medical Sciences & Peking Union of Medical College, Beijing 100050, China; 2 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China ; 3 School of Biological Sciences, University of Wollongong, NSW 2522, Australia
  • Received:2007-09-29 Online:2008-06-25 Published:2008-06-25

Abstract: Three DNA regions were sequenced for testing six fresh plant samples of Salvia species . These three DNA regions were nrDNA ITS ( nuclear ribosomal DNA internal transcribed spacer), chloroplast rpl16 ( the gene encoding ribosomal protein L16 ) , and trnL- trnF ( the cpDNA region comprising the trnL and the intergenic spacer between trnL and trnF) . The results showed that the nrDNA ITS and rpl16 genes could provide novel information for origin identification of Salvia species. Due to their higher mutation rates of these 2 gene markers, Salvia species-specific primers were designed and S. miltiorrhiza and S. yunnanensis were identified. The trnL- trnF gene expressed low mutation rate, it could not identify the species . Since the damage of DNA by the pretreatments of the dry roots of Chinese herbs, it is hard to apply the
molecular markers to commercial samples for identification.

Key words: Salvia

CLC Number: