Plant Diversity ›› 2010, Vol. 32 ›› Issue (6): 489-494.DOI: 10.3724/SP.J.1143.2010.10128

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Study on the Morphological Changes of the Ectomycorrhizae Formed by Tuber aestivum on Cyclobalanopsis glauca Seedlings

 HU  Hong-Dao-1, SU  Kai-Mei-2, CHAI  Hong-Mei-2   

  1. 1 School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan;
    2 Institute of Biotechnology & Germplasmic Resource, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
  • Received:2010-07-02 Online:2010-12-25 Published:2010-10-09


Tuber aestivum is a valuable edible symbiotic fungus. The aim of this study was to investigate the morphological changes after the ectomycorrhizae of Cyclobalanopsis glauca seedlings and Taestivum were formed. The results showed Taestivum formed bristle ectomycorrhizae on the rootlet tip with light cream color on Cglauca seedlings on the 14th day by inoculating liquid spores suspension. It formed either typical yellowish brown to brownish ectomycorrhizae with or without bristles or woolly ectomycorrhizae after inoculation in one to two months. Ectomycorrhizae were monopodial, 150-250 μm in diameter and 1-4 mm in length. The fungus mantle was 12-20 μm thick, surface smooth or with bristles, or with extramatrical hyphae. Hyphae in Hartig nets were 1-15 μm in diam. The ageing mycorrhizae became dark brown , wilted and shrunken. The yellowish green extramatrical mycelial strands could be the most important morphological character to distinguish the mycorrhizae formed by Taestivum and those formed by other Tuber species.

Key words: Tuberaceae, Ectomycorrhizae systhesis, Micro-structure, Semi-aseptic seedling, Symbiotic fungi

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