Plant Diversity ›› 2011, Vol. 33 ›› Issue (2): 225-228.DOI: 10.3724/SP.J.1143.2011.10155

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Anther Culture and Haploid Induction from Isatis indigotica (Cruciferae)


  1. Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,
    National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in
    Northwest of China, Shaanxi Normal University, Xi′an 710062, China
  • Received:2010-09-14 Online:2011-04-25 Published:2010-12-20


Anther culture and haploid induction were carried out from Isatis indigotica. The experimental results showed that cold pretreatment could increase the induction rate of callus. The induction rate of callus was 23.35% after 2 days of cold pretreatment. Then the calluses were successively transferred to differentiation medium (MS+BA 0.5mg·L-1+ NAA 1.0mg·L-1) and rooting medium (1/2MS+NAA 1.0mg·L-1). The complete plants were obtained 3 days later. Among which, those weak and green plantlets were haploids with 7 chromosomes.

Key words: Isatis indigotica, Anther culture, Haploid

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