Plant Diversity ›› 2012, Vol. 34 ›› Issue (2): 164-170.doi: 10.3724/SP.J.1143.2012.11129

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Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation

 ZHAO  Juan, CHEN  Yan, HUI  Sheng-Ming, TU  Wei-Yi, CHEN  Chen, GAO  Ju-Fang   

  1. College of Life Environment Sciences, Shanghai Normal University, Shanghai 200234, China
  • Received:2011-09-01 Online:2012-04-25 Published:2011-12-31

Abstract:

To identify new genes important for anther development, we screened for malesterile mutants among a population of Arabidopsis ecotype Columbia (Col) mutagenized by TDNA insertion (provided by ARBC). A male sterile mutant line with normal vegetative and flora development but no seed yield was isolated from Salk_118481 line. TDNA insertion site identification showed that there were no TDNA sequences in the genome of the mutants. Genetic analysis indicated that the mutant was controlled by a single recessive nuclear gene named filament no elongation because the filament of the mutant remains very short at the 13-14 stage of anther development. The fne gene was mapped to a region of 97kb between the molecular makers MBD2 and MMG4 on chromosome 5 using mapbased cloning technique. No genes involved filament elongation were reported in this region, so we believe that FNE gene could be a new gene controlling filament elongation in Arabidopsis.

Key words: Arabidopsis thaliana, Male sterile, Filament elongation, Map-based cloning

CLC Number: 

  • Q 78