Plant Diversity ›› 2012, Vol. 34 ›› Issue (5): 502-508.DOI: 10.3724/SP.J.1143.2012.12052

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ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana*

 CHENG  Zhi-Peng, CHANG  Hai-Shuang, YANG  Zhong-Nan, ZHANG  Sen   

  1. College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China
  • Received:2012-04-12 Online:2012-10-25 Published:2012-07-21


In Arabidopsis, the tapetum plays important roles in anther and pollen development by providing enzymes for callose dissolution, materials for pollenwall formation, and nutrients for microspore development. This paper describes the functional analyses of the ST273 gene in anther and pollen development by using Arabidopsis male sterile mutant st273. Mutant st273 was identified from a TDNA insertion mutant population, and genetic analysis showed that st273 mutant was controlled by a single recessive nuclear gene. A mapbased cloning approach was used, and ST273 gene was mapped to be linked to a molecular marker CIW11 on chromosome 3. Bioinformatics analysis revealed that there is a TDF1 gene near the marker CIW11. Sequencing analysis indicated that st273 mutant had a G459 to A459 basepair change in the third exon of TDF1 gene, which resulted in premature termination mutation in this region. Allelism test indicated that ST273 and TDF1 belong to the same locus. The mutant plant grows normally during the vegetative growth stage, but show developmental defects at the reproductive growth stage. Alexander staining showed that there was no pollen in the mature anther locule. Cytology observation indicated that the mutant tapetum was enlarged and vacuolated, the tetrads could not release the microspores timely, and finally no pollen was formed in the anther. These results demonstrated that ST273 protein plays an important role in tapetum and microspore development.

Key words: Arabidopsis thaliana, Male sterile, Anther development, Tapetum, ST273

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