Plant Diversity ›› 2009, Vol. 31 ›› Issue (03): 247-252.DOI: 10.3724 SP.J.1143.2009.09019

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Expression of Bioactive Chicken Alpha Interferon in Transgenic Tobacco

SONG Li1 , 2 , ZHAO De-Gang1 , 3 , WU Yong-Jun3   

  1. 1 Guizhou Key Laboratory of Agricultural Bioengineering , Guiyang 550025 , China; 2 Key Laboratory of Green Pesticide and Agricultural Biological Engineering, Ministry of Education , Guiyang 550025 , China; 3 College of Life Sciences, Guizhou University , Guiyang 550025 , China
  • Received:2009-02-04 Online:2009-06-25 Published:2009-06-25

Abstract: Expression of avian cytokine in tobacco or other plants had the economically potential use for producing pharmacological applications products and applying easily in practice . The cDNA encoding chicken alpha interferon (ChIFN-α) was introduced into Nicotiana tabacum Xanthin by Agrobacterium transformation . The transgenic plants were detected by histochemical stain assay and molecular analysis . Recombinant ChIFN-αwas confirmed by enzyme- linked immunosorbent assay (ELISA) and cytopathic effect (CPE) inhibition assay . The results showed that ChIFN-α gene was successfully introduced into tobacco plants and stably expressed at various level from 0 .0003% to 0. 0033% of total soluble protein. The biological activity of ChIFN-αwas 5 .01×103 IU􊄯g of tissue in vitro . Since interferon was involved in host antiviral response, our results provided the possibility for further study of plant oral adjuvant with ChIFN-αfor improving immunity in birds .

Key words: ChIFN-α

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