Abstract: Employing a pair of primers designed from cDNA of a HbCaM gene, two 1371 bp and 447 bp-length fragments were isolated from genome DNA in Hevea brasiliensis. Sequence analysis showed that the longer fragment was the HbCaM gene with 2 exons ( including the 77 bp and 373 bp-length fragments) and an 869 bp-length intron, and the shorter fragment had 98% identity with cDNA sequence of HbCaM gene. Compared to cDNA of the HbCaM gene, 6 mutated nucleotides were identified in the 447 bp-length fragment, in which T mutated from C at 406 bp site led to the occurrence of stop codon TAG in advance . The 447 bp- length fragment was hereby suggested to be a pseudogene of the HbCaM gene in H. brasiliensis, named HbCaMP1, which was the first pseudogene in identified H. brasiliensis geonome and the first homological pseudogene of CaM genes isolated in plant .

Key words: Hevea brasiliensis