Plant Diversity ›› 2005, Vol. 27 ›› Issue (06): 663-668.

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Effect of Kinetin (KT) on Physiological and Biochemical Variation of Cotyledons of Cucuibita pepo in Vitro during Construction of Floral Buds

HUANG Zuo-Xi1 , 2 , QING Dong-Hong1 , DUAN Hui-Guo1 , TANG Zheng-Yi1 , WANG Fang1   

  1. 1 Department of Chemistry and Life Science , Neijiang Teachers College, Neijiang 641112 , China ; 2 Flower Research Institute of Neijiang Teachers College, Neijiang 641112 , China
  • Received:2005-02-16 Online:2005-10-25 Published:2005-10-25
  • Contact: HUANG Zuo-Xi

Abstract: Physiological and chemical variation and construction of floral buds of cotyledons of Cucuibita pepo L . induced by kinetin are reported in the present paper . The results domonestrated that differentiation in floral bud of the cotyledons which cultured on MS mixed with kinetin 1 . 0 mg·L- 1 accounted for 45% of the total, whereas only reached 5% in the control . During construction of the floral buds , the endogenous soluble protein , soluble suger , RNA and POD activity of the cotyledons apparent exceeded
those of the control ; the ratio between Spd and Put (Spd􊄯Put ) is two times more than that of the control ; the content of starch and DNA are not quite different from the control , but DAP and Cad apparently decreased. It is easy to come to a conclusion that the varition of physiological and chemical induces in the cotyledons in vitro induced by kinetin promoted the construction of floral buds .

Key words: Cucuibita pepo