Plant Diversity ›› 2005, Vol. 27 ›› Issue (06): 649-652.

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Polymerziton and Electron Microscopy of Tubulin of Pollens from Hemerocallis fulva in vitro

LIAO Jun-Jie1,2 , WU Ying-Jie1 , YEN Lung-Fei1   

  1. 1 College of Biological Sciences, China Agricultural University , State Key Labratory of Plant Physiology and Biochemistry, Beijing 100094 , China ; 2 Department of Food and Bio-technology , Guangdong Light Industry Technologic College, Guangzhou 510300 , China
  • Received:2005-05-08 Online:2005-10-25 Published:2005-10-25
  • Contact: LIAO Jun-Jie

Abstract: Microtubules are the components of the cytoskeleton in all eukaryotic cells . They are essential for a wide varieties of cellular functions . Most of our knowledge of biochemistry and pharmacology of tubulin has come from extensive studies on mammalian brain microtubule systems, very little information exists on the biochemical and pharmacological properties of plant tubulin and microtubules . We developed an efficient method to prepare enough tubulin in high purity from day lily ( Hemerocallis fulva L .) pollen . Here we reported the ultrastructural morphology of microtubule polymerzied by induction of tubulin taxol in the presence of Mg2+ and GTP with transmission electron microscopy .

Key words: Hemerocallis fulva