Plant Diversity ›› 1999, Vol. 21 ›› Issue (01): 1-3.

• Articles •    

Study on Micro- isoelectric Focusing of Single Pollen Grain from Cucurbita moschata

MA Quan- Xiang,MA Quan- Shun,PANG Guang- Chang ZHAO Dong - Xu2   

  1. Department of Cell Biology, Xinxiang Medical College, Xinxiang Henan 453003
  • Online:1999-02-25 Published:1999-02-25

Abstract: Very clear electrophoresis patterns have been gotten by isoelectric focusing (IEF) and microoper-ation of single pollen grains (d≈150um) from Cucurbita moschata . The results showed that there were different protein bands among pollen grains from one flower. This segregation occurred with a classical 1: 1 Mendelian ratio by statistics in all experiments. The technique of IEF of single pollen grains and micro-oper-ation provides a new and quick screening test applied to plant genetic breeding and developmental research.

Key words: Cucurbita moschata, Single pollen grain