Plant Diversity ›› 1996, Vol. 18 ›› Issue (02): 1-3.

• Articles •    

STUDY ON CELL CULTURE IN TAXUS CHINENSIS

GAN Fan-Yuan, ZHENG Guang-Zhi, PENG Li-Ping   

  1. Kunmming Institute of Botany,Chinese Academy of Sinica,Kunming 650204
  • Online:1996-04-25 Published:1996-04-25

Abstract: Calli were induced from young stem and needle of Taxus chinensis (Pilg.) Rehd. Some basic respects of calli culture and cell suspension culture were investigated. Their capability of syn-thesis taxol were analyzed by HPLC method. Some factors of increasing the growth rate and taxol yield of cultured cells were tested. The growth rate of calli and suspension culture cells from Tax us chinensis were 0.25 g/L.d and 0.28 g / L.d (based on the cell dry weight) respectively, and their taxol content were 0.0026% and 0.012% respectively.

Key words: Taxus chinensis