Abstract: Cultural shoots derived from adult and their seedings plant of C.scabrifolia were used as experimental material. Endogenous levels of IAA, ABA and Z+ZR were determined by methed of enzymelinked immunosorbend assay(ELISA). We also probed into relation among the plant hormones mentioned above and different rooting ratio in cultured shoots derived from seedings and adult plants of C.scabrifolia. The experimental results show: The endogenous level of IAA depends upon individual line. The balance of IAA and Z+ZR during the course from seeding to adult plant is multiform, when endogenous levels of IAA increase and Z+ZR decrease, it is of benefit to induce root in cultural shoots derived from abult plant. Conversely, it is dis-advanta-geous to induce root.The endogenous level of ABA in all shoots is at lower level, and no remark-able difference between the one derived from seedings or adult plant was observed.

Key words: Crategus scabrifolia, Tissue culture