Plant Diversity ›› 1998, Vol. 20 ›› Issue (02): 1-3.
• Articles •
Liu Ji-Mei, CHEN Shan-Na, YANG Ming-Zhi,YAN Bo, HUANG Xing-Qi
Online:
Published:
Abstract: Using highly degenerate primers by RT-PCR, the conserved region in GPAT-gene was obtained from total RNA of squash (Cucurbita moschata). In the PCR reactions, the coneen- tration ratio between the degenerate primers was essential for influencing amplification efficiency. Utilizing deoxyinosine(dI) can decrease degenerate degree and enhance the specificity of PCR products and amplification efficiency.
Key words: Degenerate primer
Liu Ji-Mei, CHEN Shan-Na, YANG Ming-Zhi,YAN Bo, HUANG Xing-Qi. Application of Degenerate Primers for Amplification of the Gene Encoding Glycerol-3-phosphate Acyltransferase by Polymerase Chain Reaction[J]. Plant Diversity, 1998, 20(02): 1-3.
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URL: https://journal.kib.ac.cn/EN/
https://journal.kib.ac.cn/EN/Y1998/V20/I02/1
