Plant Diversity ›› 1992, Vol. 14 ›› Issue (03): 1-3.
• Articles •
GAN Fan-Yuan, ZHENG Guang-Zhi
Plating culture technique was used for screening high-producing of a-tocopherol variants from the culture cells of Carthamus tinctorius L. The content of α-tocopherol in cul-ture cells was measured by HPLC method. The cell variants were selected from more than 200 clones induced from single cell or small cell aggregates (2-8 cells), and found that these clones were significant difference in a-tocopherol synthesis and cell growth, a-tocopherol contents were varied from 0 to 138.9 ug/ gDW and cell growth rates were varied from 0.20 to 0.53 gDW/ l.d at the first generation of clones. There was not a significant correlation between a-tocopherol productivity and cell growth in these clones. The stability of some clones which high-producing α-tocopherol was tested. A more stable high-producing α-tocopherol clone line (CT-289) had been selected through out near 20 passages successive subculturing.
GAN Fan-Yuan, ZHENG Guang-Zhi. SCREENING OF HIGH-PRODUCING α-TOCOPHEROL CLONE LINES FROM CARTHAMUS TINCTORIUS CELL CULTURES[J]. Plant Diversity, 1992, 14(03): 1-3.
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