Abstract: Abstract The Agrobacterium strains pACK403 and pACK-404, in which the chimeric coat protein gene of tobacco mosaic virus and NPT II gene were inserted into the T-DNA region, were used to transform the leaf discs of tobacco special SRl and special G28 (haploid) with cocultivation method. The transformed discs were cultured on the medium containing sodium cefotaximatc 500mg/l and kanamycin 300mg/l to induce shoots, then the shoots were cut and cultured on the medium containing sodium cefotaximate 500 mg/l and kanamycin 100mg/l to induce roots. The results of Nopaline detection, the gene expression determination of TMV coat protein, and the resistant test of transgenic tobacco plant against TMV infection showed that this method can be reliably used to introduce foreign genes into tobacco plant and that the TMV coat protein genes have been expressed in transgenic tobacco plant. Under the strong infection condition, the transgenic tobacco plant could delay the presence of disease symptom of TMV for 4 to 25 days.

Key words: Tobacco Mosaic Virus