Abstract: Eleven ditcrpenoid alkaloids were isolated from the fresh roots of Aconitnm austroyunnanense W. T. Wang, a famous folk herbal medicine with cura tivc effects of rheumatism, apoplexy palsy, fracture, etc. Alkaloid 1, a new compound, was determined to be 14-acetyl sachaconitine on the basis of its spectroscopic data and chemical method, and named austroconitine A( 1 ). Alkaloid 2 - 8, seven known compounds, were identified as vilmorrianine B (karakoline) (2), isotalatizidine (3), vilmorrianine D (sachaconitine) (4), vilmorrianine C(5), talatisamine (6), vilmorrianine A (7), 8 -deacetyl yunaconitine ( 8 ), respectively, through the direct comparison to those authentic samples. Alkaloids 9-11 are still under identification.

Key words: Aconitum austroyunnanense