Abstract: The best medium to grow immature sunflower (Helianlhus annuus L.) cmbryos is the B, basic mcdium among MS (Murashige and Skoog, 1962), White (1963) ,B5 (1968) and Nitsch (1969) media used in our experiments. When NAA is cmployed in the immature sunflower embryos cultured in vilro, it promotes embryonic growth of the immature embryos mainly. When IAA und KT are used in combination in immature embryo culture, they have considerably regulative actionl the lower proportion of KT/IAA lnakes young embryo grow roots only while the higher proportion grow buds. Similarly, nature plant extract apparently promotes development and growth, globular embryo of sunflower can normally grow into sceedling in the medium supplemented with Bi Qi (water chestnut) Juice.

Key words: Immature embryo culture