Abstract: The purification of protoplasts from cellular and subcellular debrids by floatable washing method with a modified centrifuge tube is described. In this method a modified centrifuge tube, which possesses opened narrow and short tube at the bottom, is used. The yield of protoplasts is relatively high by means of this purification method. The average numder of protoplasts obtained from the stems of corn (Zea mays L.) was 6.2 x 10^5 gm^-1 fresh weight. The average number of protoplasts obtained from the leaves of Petunia hybrida Vilm. was 2.7x10^6 gm^-1 fresh weight. After purification the viability of plant protoplasts is high. During progress the protoplasts were cultured cell division of corn stem protoplats was observed. Cultured protoplasts of Petunia leaves underwent cell division and developed into calli. After the calli were transferred onto differential medium the roots grew.

Key words: purification of plant, protoplast