Plant Diversity ›› 2001, Vol. 23 ›› Issue (04): 1-3.

• Articles •    

The Effect of Precursor Feeding on Flavonoids Biosynthesis in Cell Suspension Cultures of Saussurea medusa

LU Dong - Ping ZHAO De - Xiu HUANG Yan ZHAO Qiao   

  1. Insituteof Botany, Chinese Academy of Sciences
  • Online:2001-08-25 Published:2001-08-25

Abstract: The effect of three precursors on flavonoids biosynthesis in cell suspension cultures of Saussurea medusa Maxim has been studied. The results show that all these precursors can promote the flavonoids biosynthesis. The optimum time for precursor feeding (OTPF) is the 6th day during the cell culture. L -pheylalanine, cinnamic acid and NaAc have the most significant promoting effect on flavonoids formation at the concentration of 0.1 mmol/L, 0.05 mmol/L and 0.1 mmol/L respectively. Of the three precursors, cinnamic acid is the most effective. The maximum flavonoids production reached 1 801 mg/L, 1.98 times as that of the control. When L - pheylalanine and NaAc were combined to be added, they have a more signifi-cant promoting effect than they were added respectively.

Key words: Sassurea medusa