Plant Diversity ›› 2001, Vol. 23 ›› Issue (04): 1-3.

• Articles •    

Cyto - Histological Studies on the Adventitious Buds Differentiating from Adventitious Roots Tips of Trichosanthes kirilowii

CHEN Hui   

  1. Biology Department, Sharud Teacher's University
  • Online:2001-08-25 Published:2001-08-25

Abstract: About 0.5 - 1 cm long adventitious root tips (with their plantlets or without) of Tnchosanthes Idr-ilowii were cultured on MS with BA 5 mg/L for 15 days, they produced numerous adventitious buds. By the paraffin - cut section method, the anatomy of adventitious buds formation was studied and the buds differenti-ation can be divided into four phases: (1) Initiation phase (0-3 days). Root meristematic cells and peri-cyclic cells first divided. (2) "The zone of transition from root to shoot " precambium formation phase (4 -6 days). The initiation cells divided and formed two or three near flat layers of cells with smaller volumes, bigger necleuses, and denser cytoplasmises. They connected and formed precambium of the transition zone from root to shoot. (3) "The transition zone from root to shoot" formation phase (7-10 days). The pre-cambium cells continualy divided at different place from the tip and made the root apex to expend, and formed half ball or a ball or shuttle in shape. In the meanwhile, root vascular tissues were transforming to shoot vascular tissues within the expanded district. (4) Buds formation phase (11-15 days).

Key words: Trichsanthes krilowii