Plant Diversity ›› 2000, Vol. 22 ›› Issue (03): 1-3.

• Articles •    

Sequence Comparison of Part cDNAs Coding for Glycerol-3- Phosphate Acyltransfera from Rice Strains with Different Chilling-Resistance

LIU Ji -Mei, CHEN Shan- Na, YAN Bo, HUANG Xing -Qi, YANG Ming- Zhi    

  1. Biology Department, Yunnan University, Kunming 650091, China
  • Online:2000-06-25 Published:2000-06-25

Abstract: Using RT- PCR technique, we obtained a fragment of cDNA about 315bp from rice strains with different chilling- resistance. In amplification reactions, the used primers are degenerated primeis, which were designed according to the conserved amino acid sequences of glycerol - 3 - phosphate acyltransferase from several kinds of dicotyls. Sequences obtained show that the cDNAs fragments all have the parts of cD-NAs coding for Glycerol - 3 - phosphate acyltransferase, contain 315 nucleotides and encode 105 amino acids. While the cDNAs sequences and deduced amino acids sequences from the rice strains with different chilling- resistance were different from each other. The bigger difference of chilling - resistance between strains is, the bigger differences between their cDNAs sequences and the deduced amino acids sequences are. The difference of chilling-resistance is probably related to the subtitution of proline.

Key words: Glycerol -3- phosphate acyltransferase (GPAT)