Abstract: Carnation (Dianthus caryophyllus) in 3 production models growing in Kunming was investigated, and 146 samples were collected for experiments. The samples were inspected by ELISA and SEM to identify whether they were infected by carnation virus. The results indicated the main prevalenting carnation viruses in Kunming region are carnation mottle virus and carnation necroflect virus. Using carnation variety "Qiaoxinlang" as test material, which infected by caarnation mottle virus, we studies the differences of devirus rate and survive rate by using 3 different methods, i.e.direct meristem-tip culture, meristem-tipculture in combination with thermotherapy, and meristem - tip culture in combination with Ribavirini.

Key words: Carnation