Development of Molecular Markers Used to Identify Two Types of Fragrant Rice and Analysis of Mutation Sites of BADH2 Gene in 24 Varieties of Fragrant Rice

doi:10.3724/SP.J.1143.2011.11019

Abstract

Abstract:

Functional molecular markers M7 and M2 have been developed based on the DNA sequence differences of badh2 between fragrant rice varieties and nonfragrant varieties in intron2, intron 4, exon7 and exon 2 respectively. PCR analyses on genome DNA of exon7mutated fragrant rice Wxiang 99075, exon2mutated fragrant rice Wuxiang14,nonfragrant rice 261S and the F1 plants by M7 and M2 showed that M7 and M2 could be absolutely used to the molecular markerassisted rice breeding experiments when exon7mutated and exon2mutated fragrant rice varieties are used as parents. The design of M7 primers took mutations both in exons and intrones into account. Moreover, taking 261S,Wxiang 99075 and Wuxiang14 as controls, the mutation sites of badh2 in 22 fragrant rice varieties were analyzed, it was showed that fragrant rice varieties could be classified into 3 types: exon 2mutated fragrant rice, exon 7mutated fragrant rice and nonexonmutated fragrant rice. At the same time, the mutation sites of badh2 in the main fragrant rice varieties which are grown in Shanghai and the surrounding areas have been verified. This research laid an important foundation for molecular markerassisted selection for novel fragrant rice.

Key words: Fragrant rice, BADH2 gene (badh2), Functional molecular marker, Mutation site analyses

CLC Number:

Q 75

XU Xiao-Long, ZHAO Guo-Chao, LI Jian-Yue. Development of Molecular Markers Used to Identify Two Types of Fragrant Rice and Analysis of Mutation Sites of BADH2 Gene in 24 Varieties of Fragrant Rice[J]. Plant Diversity, 2011, 33(6): 667-673.

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