Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae)

doi:10.3724/SP.J.1143.2012.12028

Abstract

Abstract:

A 4231bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques. The result of alignment and structure prediction of DNA sequences showed that one 1194bp nucleotides gene ASRcb1 encoding B mating pheromone receptor was found, including four introns(72bp, 49bp, 48bp and 41bp) and five extrons (217bp, 113bp, 67bp, 138bp and 449bp). The spliced open reading frame (ORF) contains 984bp nucleotides encoding 327 amino acid residues, and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors. The genetic evolution of pheromone receptor showed ASRcb1 was clustered with more receptors, which suggested multiple ways of evolution occurred in fungi.

Key words: Agrocybe salicacola, Pheromone receptor, Mating type, Degenerate PCR, Amino acid sequence

CLC Number:

Q 75

CHEN Wei-Min-, CHAI Hong-Mei-, ZHANG Xiao-Lei-, LI Shu-Hong-, ZHAO Yong-Chang- . Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae)[J]. Plant Diversity, 2012, 34(5): 519-524.

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