The Activity of Principal Antioxidant Enzymes and the Content of Metabolites in Dormancy Breaking and Germination of Davidia involucrate Seeds

doi:10.7677/ynzwyj201515074

Abstract

Abstract:

The principal antioxidant enzymes and metabolites were reported to play a very important role in eliminating reactive oxygen species and maintaining vigour in dormancy breaking and germination of Davidia involucrate seeds. However, the researches on the change laws of content and activity are seldom. In present study, we chose Davidia involucrate seeds which collected from the Mabian Nature Reserve as experimental materials, and then investigated the activity of principal antioxidant enzymes and the content of metabolites in dormancy breaking and germination of the seeds. The results showed that the activity of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) exhibited an increasing trend but the activity of total antioxidant capacity (TAOC) decreased greatly in early stage of stratification. Comparing with the decrease of protein (Pr), the content of ascorbic acid (Vc) and free amino acid (FAA) significantly increased. In middle stage of stratification, the activity of SOD and TAOC increased firstly and then decreased at last. The activity of POD always maintained a higher level although the activity of CAT and GR decreased greatly. In terminal stage of stratification, the activities of POD, GR, TAOC and the content of FAA and Vc exhibited an increasing trend, while the activity of CAT and SOD maintained a low level. In germination stage, the activity of all antioxidant enzymes and the content of Vc and FAA were increased obviously (P < 005), but the content of the protein was decreased. These results suggested that Davidia involucrata seeds may have a good ability to efficiently remove the effects on cell structure caused by reactive oxygen species (ROS) by keeping the interaction of antioxidant enzymes and metabolites successively during the period of dormancy breaking and germination. This adjustment helps to ensure the germination of seeds by acceleration the postripening in morphological and physiological of progresses.

Key words: Davidia involucrata seeds, Dormancy, Germination, Antioxidant enzymes, Metabolites

CLC Number:

Q 945.3

YANG Yan-, LI Yun-Xiang-, XU Xiao. The Activity of Principal Antioxidant Enzymes and the Content of Metabolites in Dormancy Breaking and Germination of Davidia involucrate Seeds[J]. Plant Diversity, 2015, 37(06): 779-787.

References

李合生, 2000. 植物生理生化实验原理和技术［M］. 北京: 高等教育出版社, 164—165, 192—194, 258—260
聂剑初, 2000. 生物化学简明教程［M］. 北京: 高等教育出版社
宋松泉, 程红炎, 姜孝成, 2008. 种子生物学［M］. 北京: 科学出版社, 224
增田芳雄, 1980. 植物生理学的二大先驱者——Sachs和Pfeffer［J］. 植物生理学通讯, (2): 43—50
Bai YF (白永富), Lu XP (卢秀萍), 2006. The correlation between the soluble protein content and the free amino acids during germination of Tobacco seeds［J］. Chinese Agricultural Science Bulletin (植物生理科学), 22 (8): 286—288
Baskin JM, Baskin CC, 2004. A classification system for seed dormancy［J］. Seed Science Research, 14 (1): 1—16
Chen G (陈刚), Wang GZ (王广志), Liu XL (刘秀丽) et al., 2002. Effects of various treatments on germination and catalase activity of tobacco seeds［J］. Tobacco Science & Technology (烟草科技), 2 (2): 11—14
Chen KR (陈坤荣), 1988. The dormancy of Davidia involucrata seeds［J］. Plant Physiology Communication (植物生理学通讯), 25 (3): 24—28
Galston AW, Lavee S, Siegel BZ, 1968. The induction and repression of peroxidase isozymes by 3indoleacetic acid in: “ Biochemistry and physiolopy of Plant Growth Substarces”(F. Wightman and G. Setterfield eds ) ［C］. Proceedings of the 6th International Conference on. Plant Growth Substances held at Carleton University Ottawa, 455—472
Gu JT (谷建田), Kong XH (孔祥辉), Chen H (陈杭), 1993. Hydrationdehydration treatment for pre and postaging Lycopersicon esculentum Mill Seeds: physiological and biochemical changes［J］. Acta Phytophysiologica Sinica (植物生理学通报), 19 (2): 131—136
Guo LH (郭丽红), Chen SN (陈珊娜), Gong M (龚明), 2002. Effect of calcium on glutathione reductase of maize seedlings［J］. Plant Physiology Communication (植物生理学通讯), 38 (2): 115—117
Han KJ (韩克杰), Sun X (孙霞), Xing SY (邢世岩) et al., 2006. Studies on physiological characteristics of Corylus avellana L. during storage and germination［J］. Journal of Wuhan Botanical Research (武汉植物学研究), 24 (5): 435—440
He JX (何军贤), Fu JR (傅家瑞), 1996. The research progresses in Lea proteins of seeds［J］. Plant Physiology Communication (植物生理学通讯), 32 (4): 241—246
Huang CL (黄承玲), Gao GL (高贵龙), Chen X (陈训) et al., 2010. Physiological changes of Rhododendron agastum Balf. f. et W. W. Sm during seed germination［J］. Seed (种子), 29 (5): 94—96
Huang YJ (黄永菊), Wu XM (伍晓明), Sheng JX (沈金雄) et al., 2000. Research on ultradry storage of soybean seeds［J］. Chinese Journal of Oil Crop Sciences (中国油料作物学报), 22 (3): 39—42
Kahn AA, 1989. Seed Dormancy and Germination of Physiological and Biochemical［M］. Beijing: Agricultural Press
Ke DS (柯德森), Sun GC (孙谷畴), Wang AG (王爱国) et al., 2003. Relationship between ascorbic acid and germination of seeds［J］. Chinese Journal of Applied & Environmental Biology (应用与环境生物学报), 9 (5): 497—500
Koornneef M, Bentsink L, Hilhorst H, 2002. Seed dormancy and germination［J］. Current Opinion in Plant Biology, 5 (1): 33—36
Lei NF (雷泞菲), Peng SM (彭书明), Niu B (牛蓓) et al., 2009. Changes on edogenous phytohormone content of the Davidia involucrate seed during dormancy and germination process［J］. Guihaia (广西植物), 29 (1): 66—69
Li X (李雄), Li BZ (李秉真), Wu Y (乌云) et al., 1996. Physiological studies on seed dormancy and postripening of Crataegus shanguinea Pall［J］. Journal of Inner Mongolia Institute of Agriculture & Animal Husbandry (内蒙古农牧学院学报), 17 (4): 56—60
Li JY (李俊珏), Xu X (胥晓), Yang P (杨鹏) et al., 2012. Effects of aluminum stress on ecophysiological characteristics of male and female Populus cathayana seedlings［J］. Chinese Journal of Applied Ecology (应用生态学报), 23 (1): 45—50
Li YX (黎云祥), Su ZX (苏智先), Chen F (陈放), 2002a. Rapid extraction of gnomic DNA from leaves and bracts of dove tree (Davidia involucrata) ［J］. Plant Molecular Biology Reporter, 20 (2): 185a—185e
Li YX (黎云祥), Chen L (陈利), Lin J (林娟) et al., 2002b. Suppression subtractive hybridization cloning of cDNAs of differentially expressed genes in dovetree (Davidia involucrata) bracts［J］. Plant Molecular Biology Reporter, 20 (3): 231—238
Li YX (黎云祥), 2003. Present state and perspectives of the biological study on Davidia involucrate［J］. Journal of China West Normal University (Natural Sciences) (西华师范大学学报: 自然科学版), 24 (3): 269—275
Li YX, 2003. Cloning, sequence analysis, and prokaryotic expression of cDNA encoding a putative nonspecific lipidtransfer protein from the bracts of Dovetree (Davidia involurate baill.) ［J］. Journal of Plant Biology, 46 (3): 167—172
Liang XH (梁新华), Zhang MZ (张明哲), Gao JX (高金霞), 2004. Effect of drought stress on germination and soluble protein content of glycyrrhiza glabra I. and glycyrrhiza inflata seeds during the process of primary germination［J］. Ningxia Journal Agriculture and Forestry Science and Technology (宁夏农业科技), (4): 6—7
Lu CM (陆长梅), Wu GR (吴国荣), Zhou CF (周长芳) et al., 2000. The effects of illumination on plant SOD activity［J］. Journal of Nanjing Normal University (Natural Science) (南京师大学报: 自然科学版), 23 (3): 96—99
Luo P (罗培), Zhou SL (周申立), 2007. Effect of land use on ecological benefit of farm belt in suburbs［J］. Journal of Ecology and Rural Environment (生态与农村环境学报), 23 (4): 6—10
Ma GY (马国英), Xu XZ (徐锡忠), Zhang CL (章崇玲), 1991. Membrane in the process of seed aging changes and the reasons［J］. Plant Physiology Communication (植物生理学通讯), 27 (3): 233—236
Pang FH (庞发虎), Du JJ (杜俊杰), Liu F (刘飞), 2004. Study on changes of activity in antioxidate system enzyme in shoots and buds of dormant apricots［J］. Journal of Hubei Institute for Nationalities (Natural Science Edition) (湖北民族学院学报: 自然科学版), 22 (4): 27—30
Philippi T, 1993. BetHedging germination of desert annuals: beyond the first year［J］. American Naturalist, 142 (3): 474—487
Richter G, 1985. Plant Metabolism［M］. Beijing: Science Press
Robert CH, 1973. Oxidative process and the control of seed germination in “seed ecology” ［C］. London: Butterworth, 189
Ruan Y (阮英), Liu KL (刘开朗), Shen L (申琳) et al., 2006. Metabolism of dynamic changes of the reactive oxygen in tomato pericarp and seed tissues during fruit ripening and senescence［J］. Acta Horticulturae Sinica (园艺学报), 33 (1): 63—67
Su ZX (苏智先), Zhang SL (张素兰), 1999. The reproductive phenology and the influencing factors of Davidia involucrata population［J］. Journal of Sichuan Teachers College (Natural Science) (四川师范学院学院: 自然科学版), 20 (4): 313—318
Sun Y (孙艳), Cui HW (崔鸿文), Yin MA (尹明安) et al., 2006. The effects of accorbic acid on cucumber seeds germination and the growth of Jin 431 cucumber tissue cultured seedlings［J］. Journal of Hebei Agrotechnical Teachers College (河北农业技术师范学院学报), 10 (4): 72—74
Tian LL (田莉莉), Fang JB (方金豹), Wang LR (王力荣) et al., 2006. Changes of several physiological indexes during dormancy releasing in nectarine cultivar Huaguang［J］. Journal of Fruit Science (果树学报), 23 (1): 121—124
Wan CK (万朝坤), 1988. Anatomical study on seed dormancy of DoveTree (Davidia involucrate) ［J］. Journal of CentralSouth Forestry College (中南林学院学报), (1): 35—39
Wang H (汪海), Zhang SL (张素兰), Su ZX (苏智先) et al., 2007. The effects of accelerated aging on fargesia Denudata (Gramineae) seedsand the physiological differences among seeds of different sizes［J］. Acta Botanica Yunnanica (云南植物研究), 29 (6): 677—681
Wang HH (王海华), Jiang MY (蒋明义), Kang J (康健) et al., 2003. Relationship between seed germination and active oxygens metabolism in maize treated with low nickel concentration［J］. Acta Agronomica Sinica (作物学报), 29 (4): 601—605
Wang J (王娟), Li DQ (李德全), 2001. The accumulation of plant osmoticum and activated oxygen metabolism under stress［J］. Chinese Bulletin of Botany (植物学通报), 18 (4): 459—465
Wang JH (王建华), Liu HX (刘鸿先), 1989. The role of superoxide dismutase (SOD) in stress physiology and senescence physiology of plant［J］. Plant Physiology Communications (植物生理学通讯), (1): 1—7
Wang SY, Faust M, 1994. Changes in the antioxidant system associated with budbreak in ‘Anna’ apple (Malus domestica Borkh.) buds［J］. J Amer Soc Hort Sci, 119 (4): 735—741
Wang XP (王献溥), Li JQ (李俊清), Zhang JX (张家勋) et al., 1995. Bioecological characteristics and cultivation of the Dove tree in China［J］. Guihaia (广西植物), 15 (4): 347—353
Wu LG (武禄光), 1987. Preliminary research on function of peroxidase in Acer mono seed dormancy to germination［J］. Journal of Northeast Forestry University (东北林业大学学报), 15 (6): 9—14
Wu NB (吴能表), Wang TJ (王图锦), 2006. Dynamic changes of physiological indexes in the seed budding of Raphanus sativus L［J］. Journal of Southwest China Normal University (Natural Science) (西南师范大学: 自然科学版), 31 (6): 117—120
Yang YZ (杨玉珍), Li SP (李生平), Wu QX (吴青霞) et al., 2006. The dynamic changes of proteins and activities of nitrogen metabolism enzymes in Ginkgo biloba seeds during germination［J］. Journal of Nanjing Forestry University (Natural Sciences Edition) (南京林业大学学院: 自然科学版), 30 (4): 119—122
Yin HJ (尹华军), Liu Q (刘庆), 2004. Advances in studies on molecular biology of seed dormancy and germination［J］. Chinese Bulletin of Botany (植物学通报), 21 (2): 156—163
Zeng SX (曾韶西), Wang YR (王以柔), Liu HX (刘鸿先), 1987. The effect of chilling stress on the content of ascorbic acid in rice seedlings［J］. Acta Phytophysiologica Sinica (植物生理学报), 13 (44): 365—370
Zhou XL (周小理), Cheng SN (成少宁), Tang W (唐文) et al., 2010. Studies on antioxidant enzyme activities in buckwheat during seed germination［J］. Industrial Microbiology (工业微生物), 40 (4): 53—56
Zhu C (朱城), Fang ZN (房正浓), Zheng GW (增广文), 2000. The effect of HVEF treatment on lipid peroxidation of aged cucumber seeds［J］. Journal of Zhejiang University (Agric. & Life Sci.) (浙江大学学报: 农业与生命科学版), 26 (2): 127—130
Zhu LJ (朱利君), Su ZX (苏智先), Hu JY (胡进耀) et al., 2009. Peroxidase activity and MDA content of endangered species Davidia involucrate［J］. Chinese Journal of Ecology (生态学杂志), 28 (3): 451—455
Zhu X (朱翔), Sun LH (孙丽华), 2005. A study of peroxidase activity in the process of seed germination of Phoebe Nees［J］. Journal of Sichuan Forestry Science and Technology (四川林业科技), 26 (2): 46—49
Zhuang WJ (庄伟健), Zhuang B (庄彪), Zhang SB (张书标) et al., 2002. Physiological foundation research of medicament treatment storage techniques for peanut seedsⅠ. Effect of medicament treatment on seed vigor and radical content［J］. Journal of Peanut Science (花生学报), 31 (2): 1—6

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