%A HU Ting-Zhang , , HUANG Xiao-Yun , XIAO Guo-Sheng , QV Xiao-Xiao %T Molecular Cloning, Expression and Activity Analysis of a Glutathione S-transferase in Rice %0 Journal Article %D 2008 %J Plant Diversity %R 10.3724 SP.J.1143.2008.08038 %P 688-692 %V 30 %N 6 %U {https://journal.kib.ac.cn/CN/abstract/article_30053.shtml} %8 2008-12-25 %X OsGSTL1 is a glutathione S-transferase (GSTs , EC 2. 5 . 1 . 18 ) , lambda class gene in chromosome 3 of rice ( Oryza sativa L .) . It includes 8 introns and 9 exons, and encodes a protein of 243 amino acid residues . The OsGSTL1 gene was cloned into pYTV vector and was transformed into yeast strain PEP4 .Western blot analysis showed the exogenous OsGSTL1 was expressed in transformed yeast . GST activity of crude extracts of yeast showed the OsGSTL1 transgenic yeast had higher levels of GST activities than control yeasts, which demonstrate the OsGSTL1 has glutathione S- transferase activity .