%A Hu Zhong %T THE GLUCOSINOLATES AND THIOGLUCOSIDASE OF CAPPARIS MASAIKAI SEEDS %0 Journal Article %D 1988 %J Plant Diversity %R %P 1-3 %V 10 %N 02 %U {https://journal.kib.ac.cn/CN/abstract/article_32132.shtml} %8 1988-04-25 %X A new glucosinolate, the 2-hydroxyethylglucosinolate was isolated and identified from seeds of Capparis masaikai Levl, with a yield of 3% of dry seed meal. Its enzymatic product at pH 6.0 was oxazolidine- 2 -thione. The thioglucosidase was extracted from seed meal powder with 1 N Nad, precipitated with ammonium sulfate, and futher purified by gel filtration on Sephadex G50 and being passed through a CM-cellulose column. The purified thioglucosidase was showed to be of Mr =116 kD, pI =5.05, consisted of two near equal subunits. It differs from the thioglucosidases isolated from the seeds of Cruciferae, which are glyco proteins with Mr = 140 kD and a carbohydrate content of 15%, in that it was showed not to contain carbohydrate.