%A XU Li-Xia, WU Xiao-Lu, TANG Xiao-Qian, YAN Bo %T Effect of New Specificity and Yield Enhancer on Amplification of lea 3 Gene %0 Journal Article %D 2010 %J Plant Diversity %R 10.3724/SP.J.1143.2010.10069 %P 535-538 %V 32 %N 6 %U {https://journal.kib.ac.cn/CN/abstract/article_32918.shtml} %8 2010-12-25 %X

Amplification of a target DNA by the polymerase chain reaction (PCR) often requires experimental optimization factors especially when the target sequence contains high content of GC. Dimethyl sulfoxide, formamide, glycerol, NP40 and Tween20 are known to increase, the specificity and efficiency of PCR under certain conditions. In the amplification of lea3 gene in Gramineae, we found a new Extreme Thermostable SingleStranded DNA Binding Protein (ET SSB) and improved yield of PCR. Addition of 200 ng of ET SSB per 50 μl reation decreased the formation of nonspecific DNA fragments and increased the yield of specific PCR products.