%A XU Li-Xia, WU Xiao-Lu, TANG Xiao-Qian, YAN Bo %T Effect of New Specificity and Yield Enhancer on Amplification of lea 3 Gene %0 Journal Article %D 2010 %J Plant Diversity %R 10.3724/SP.J.1143.2010.10069 %P 535-538 %V 32 %N 6 %U {https://journal.kib.ac.cn/CN/abstract/article_32918.shtml} %8 2010-12-25 %X
Amplification of a target DNA by the polymerase chain reaction (PCR) often requires experimental optimization factors especially when the target sequence contains high content of GC. Dimethyl sulfoxide, formamide, glycerol, NP40 and Tween20 are known to increase, the specificity and efficiency of PCR under certain conditions. In the amplification of lea3 gene in Gramineae, we found a new Extreme Thermostable SingleStranded DNA Binding Protein (ET SSB) and improved yield of PCR. Addition of 200 ng of ET SSB per 50 μl reation decreased the formation of nonspecific DNA fragments and increased the yield of specific PCR products.