%A ZHAO Juan, CHEN Yan, HUI Sheng-Ming, TU Wei-Yi, CHEN Chen, GAO Ju-Fang %T Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation %0 Journal Article %D 2012 %J Plant Diversity %R 10.3724/SP.J.1143.2012.11129 %P 164-170 %V 34 %N 2 %U {https://journal.kib.ac.cn/CN/abstract/article_33048.shtml} %8 2012-04-25 %X

To identify new genes important for anther development, we screened for malesterile mutants among a population of Arabidopsis ecotype Columbia (Col) mutagenized by TDNA insertion (provided by ARBC). A male sterile mutant line with normal vegetative and flora development but no seed yield was isolated from Salk_118481 line. TDNA insertion site identification showed that there were no TDNA sequences in the genome of the mutants. Genetic analysis indicated that the mutant was controlled by a single recessive nuclear gene named filament no elongation because the filament of the mutant remains very short at the 13-14 stage of anther development. The fne gene was mapped to a region of 97kb between the molecular makers MBD2 and MMG4 on chromosome 5 using mapbased cloning technique. No genes involved filament elongation were reported in this region, so we believe that FNE gene could be a new gene controlling filament elongation in Arabidopsis.