%A HUA Ping-, Lv-Hu-, Yu-Ji-Gong-, Leng-He-Ping-, Jiang-Xian-You-, Hua-Dong %T Effects of Different Culture Conditions on Cell Growth and Theanine Biosynthesis in Suspension Cells of Camellia sinensis ( Theaceae) %0 Journal Article %D 2006 %J Plant Diversity %R %P 215-218 %V 28 %N 2 %U {https://journal.kib.ac.cn/CN/abstract/article_33254.shtml} %8 2006-04-25 %X

The suspension culture of callus obtained from tender leave of Camellia sinensis was conducted withMS mediumplus IBA 2 mgPL and 6-BA 4 mgPL in a fermenter. The effects of different factors on the growth of tea cells and the amount
of synthesized theaninewere analyzed. The results showed that both the tea- cell growth and theanine accumulation reachedtheir peak period from 19 to 22 days after culture. Both growth of the cells and the amount of theanine reached optimal va-lues when the medium contained NH4+PNO3- 110P601 0 mmolPL, K+ 10010 mmolPL, Mg2+ 310 mmolPL, H2 PO4- 310mmolPL, sucrose 3010 gPL and protein 210 gPL. Both the logarithmic and stationary phases were enhanced when theamount of sucrose and protein were raised in the medium. Alteration of H2PO4- concentration in the medium had an effecton the simultaneity of the cel-l growth and theanine accumulation. The activity of theanine- synthatase ( L-glutamate: ethy-lamine lingase) can be affected by K+ andMg2+ . Accumulation of theanine could be greatly raised when ethy-l amine wassupplemented in the medium. The efficiency of ethy-l amine on the accumulation of theanine is related to the addition methodi1 e. sequential supplement of ethy-l amine into the medium was better than single addition of that.