%A YAN Li-Jun, ZHANG Zhi-Rong, LI De-Zhu, GAO Lian-Ming %T Isolation and Characterization of Microsatellite Markers for the Chinese Endemic Species Rhododendron spinuliferum (Ericaceae) %0 Journal Article %D 2014 %J Plant Diversity %R %P 41-46 %V 36 %N 01 %U {https://journal.kib.ac.cn/CN/abstract/article_33284.shtml} %8 2014-01-25 %X
Rhododendron spinuliferum (Ericaceae) is an ornamental and medicinal plant endemic to southwest China. In order to study hybridization between Rspinuliferum and Rspiciferum, a FIASCO (Fast Isolation by AFLP of Sequences Containing Repeats) method was used to develop microsatellite markers in Rspinuliferum. A total of 28 microsatellite markers were isolated from 100 SSR primer pairs, of which 22 were polymorphic. Polymorphism of the 22 polymorphic loci was assessed separately in 24 individuals collected from two wild populations. The number of alleles per locus ranged from 2 to 5, with an average of 34, while observed (HO) and expected (HE) heterozygosities varied from 0083 to 0792 and from 0153 to 0744, respectively. The same 28 microsatellite markers were also tested in two wild populations (12 individuals from each) of Rspiciferum. Twenty two of the markers were successfully amplified, of which 20 were polymorphic. Estimates of diversity in two natural populations of Rspiciferum based on the 20 polymorphic markers revealed that number of alleles per locus ranged from 2 to 6, with a mean of 32, while observed (HO) and expected (HE) heterozygosities ranged from 0000 to 0833 and from 0117 to 0736, respectively. These newly developed microsatellite markers will be used in future studies of hybridization and the population genetics of Rspinuliferum and its closely related species.