%A YU Jiao-Jun, PAN Li, PAN Yue-Zhi, GONG Xun %T Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae) %0 Journal Article %D 2014 %J Plant Diversity %R 10.7677/ynzwyj201413109 %P 219-226 %V 36 %N 02 %U {https://journal.kib.ac.cn/CN/abstract/article_33305.shtml} %8 2014-03-25 %X
Three types of intermediate morphological individuals were found in the sympatric distribution areas of Ligularia vellerea and Lsubspicata. These intermediate individuals were hypothesized to be the hybrids of these two species based on the detailed comparison of their morphological characters. To test this hypothesis, we compared the DNA sequence of Lvellerea, Lsubspicata and the intermediate individuals by using the internal transcribed spacer region of nuclear ribosomal and atpBrbcL intergenic spacer region of the chloroplast. Direct sequencing of ITS clearly had additives and then cloned sequencing cloned out two kinds of sequences that were identical to those putative parental species, thus these putative hybrids were approved. With different morphological characters among three types, their plastid donors were all Lsubspicata according to the maternal sequences of atpBrbcL.