长柔毛野豌豆编码甘油-3-磷酸酰基转移酶基因的克隆及序列分析

Plant Diversity ›› 2002, Vol. 24 ›› Issue (05): 1-3.

• 研究论文 •

长柔毛野豌豆编码甘油-3-磷酸酰基转移酶基因的克隆及序列分析

吕燕波　陈善娜　鄢波杨明挚黄兴奇　　

成都军区昆明总医院检验科

出版日期:2002-10-25 发布日期:2002-10-25

Cloning and Nucleotide Sequence of cDNA for the Glycerol-3-phophate Acyltsansferase from Vicia villosa

Lu Yan-Bo1,CHEN Shan-Na1,YAN Bo2,YAHG Ming-Zhi1, HUANG Xing-Qi2

1 Biology Department, Yunnan Uniuersity. Kunming 650091,China; 2 lnstitute of Biotechnology,Yunnan Academy of Agricultural Sciences,Kunming 650223,china

Online:2002-10-25 Published:2002-10-25

摘要/Abstract

摘要： 甘油-3-磷酸酰基转移酶(GPAT)基因与植物抗冷性密切相关.克隆到的长柔毛野豌豆(Vicia villosa)GPAT基因的编码区完整的cDNA片段长1377bp,编码458个氨基酸残基,与蚕豆(Vicia faba)和豌豆(Pissum sativum)比较,其核苷酸序列的同源性分别为94.1%和93.3%,氨基酸序列的同源性分别为96.9%和98.0%.

关键词: 长柔毛野豌豆, 甘油-3-磷酸酰基转移酶, cDNA, 植物抗冷性

Abstract: Glycerol-3-phosphate acyltransrerase (GPAT)gene is closely relative to the chilling-resistance of piants. We isolated the cDNA encoding the GPAT from Vicia uillosa cotyiedons. The cDNA fragement includes the whole coding region,which contains 1377 base pairs and codes 458 amino acids. Comparing to Vicia faba and pissum satiuum,the cDNA sequece and the deduced amino acid sewuence of Vicia uil-losa GPAT gene habe 94.1% and 93.3%, 96.9%and 98.0% homology respectively.

Key words: Vicia uillosa

吕燕波　陈善娜　鄢波杨明挚黄兴奇　　. 长柔毛野豌豆编码甘油-3-磷酸酰基转移酶基因的克隆及序列分析[J]. Plant Diversity, 2002, 24(05): 1-3.

Lu Yan-Bo,CHEN Shan-Na,YAN Bo,YAHG Ming-Zhi, HUANG Xing-Qi. Cloning and Nucleotide Sequence of cDNA for the Glycerol-3-phophate Acyltsansferase from Vicia villosa[J]. Plant Diversity, 2002, 24(05): 1-3.

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长柔毛野豌豆编码甘油-3-磷酸酰基转移酶基因的克隆及序列分析

Cloning and Nucleotide Sequence of cDNA for the Glycerol-3-phophate Acyltsansferase from Vicia villosa

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