Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

doi:10.1016/j.pld.2020.04.003

Plant Diversity ›› 2020, Vol. 42 ›› Issue (05): 323-333.DOI: 10.1016/j.pld.2020.04.003

• Articles • 下一篇

Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

Yi-Jun Qiao^a,b, Cheng-Zhen Gu^a,c, Hong-Tao Zhu^a, Dong Wang^a, Meng-Yue Zhang^d, Yi-Xuan Zhang^d, Chong-Ren Yang^a, Ying-Jun Zhang^a,e

a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, China;
b University of Chinese Academy of Sciences, Beijing, 100049, China;
c Fujian Agriculture and Forestry University, Fuzhou, 350002, China;
d School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China;
e Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

收稿日期:2019-10-07 修回日期:2020-04-17 出版日期:2020-10-25 发布日期:2020-10-28
通讯作者: Dong Wang, Ying-Jun Zhang
基金资助:
The authors are grateful to the members of the Analytical Group in State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, for measuring all the spectra. We also appreciate Prof. Wei-Qi Li, Dr. Yan-Xia Jia and Dr. Xing Huang for providing materials and technical support on plant bioassays. This work is supported by the Science and Technology Planning Project (2013FC008) and the Major Science and Technique Programs (2016ZF001-001) of Yunnan Province, China, and YungChi Cheng academician workstation of Yunnan provincial academy of science and technology (2015IC017).

Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

Yi-Jun Qiao^a,b, Cheng-Zhen Gu^a,c, Hong-Tao Zhu^a, Dong Wang^a, Meng-Yue Zhang^d, Yi-Xuan Zhang^d, Chong-Ren Yang^a, Ying-Jun Zhang^a,e

a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, China;
b University of Chinese Academy of Sciences, Beijing, 100049, China;
c Fujian Agriculture and Forestry University, Fuzhou, 350002, China;
d School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China;
e Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

Received:2019-10-07 Revised:2020-04-17 Online:2020-10-25 Published:2020-10-28
Contact: Dong Wang, Ying-Jun Zhang
Supported by:
The authors are grateful to the members of the Analytical Group in State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, for measuring all the spectra. We also appreciate Prof. Wei-Qi Li, Dr. Yan-Xia Jia and Dr. Xing Huang for providing materials and technical support on plant bioassays. This work is supported by the Science and Technology Planning Project (2013FC008) and the Major Science and Technique Programs (2016ZF001-001) of Yunnan Province, China, and YungChi Cheng academician workstation of Yunnan provincial academy of science and technology (2015IC017).

摘要/Abstract

摘要： Panax notoginseng (Araliaceae) is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years. In recent years P. notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation. In this study, we aim to explore the allelochemicals of P. notoginseng and their interactions with various plants and rhizosphere microorganisms. The chemical constituents of the soil cultivated with 3-year-old P. notoginseng were studied by column chromatography, spectroscopic and GC-MS analyses. We identified 13 volatile components and isolated six triterpenes (1-4, 6-7) and one anthraquinone (5). Compounds 1-7 were tested for their effects on seed germination and root elongation in P. notoginseng, corn, wheat, turnip, water spinach and Arabidopsis thaliana. We also examined the effect of compounds 1-7 on the growth of ten rhizosphere microorganisms of P. notoginseng. At a concentration of 1.0 μg mL^-1, compounds 3 and 5-7 caused the death of P. notoginseng root cells and compounds 2, 6 and 7 induced the death of root cells of A. thaliana. Compounds 1-5 and 7 inhibited elongation of A. thaliana root tip cells at a concentration of 10.0 μg mL^-1. Moreover, at a concentration of 0.1 mg mL^-1, compounds 3, 4, 6 and 7 inhibited the growth of probiotics and promoted the growth of pathogens of P. notoginseng. These results suggest that these isolated ursane-type triterpenoid acids and anthraquinone are potential allelochemicals that contribute to continuous-cropping obstacles of P. notoginseng.

关键词: Panax notoginseng, Continuous cropping obstacle, Allelochemical, Triterpenes, Anthraquinone

Abstract: Panax notoginseng (Araliaceae) is an important ginseng herb with various health benefits and a history of cultivation in southwestern China over 400 years. In recent years P. notoginseng has faced serious continuous-cropping obstacles due to its large-scale cultivation. In this study, we aim to explore the allelochemicals of P. notoginseng and their interactions with various plants and rhizosphere microorganisms. The chemical constituents of the soil cultivated with 3-year-old P. notoginseng were studied by column chromatography, spectroscopic and GC-MS analyses. We identified 13 volatile components and isolated six triterpenes (1-4, 6-7) and one anthraquinone (5). Compounds 1-7 were tested for their effects on seed germination and root elongation in P. notoginseng, corn, wheat, turnip, water spinach and Arabidopsis thaliana. We also examined the effect of compounds 1-7 on the growth of ten rhizosphere microorganisms of P. notoginseng. At a concentration of 1.0 μg mL^-1, compounds 3 and 5-7 caused the death of P. notoginseng root cells and compounds 2, 6 and 7 induced the death of root cells of A. thaliana. Compounds 1-5 and 7 inhibited elongation of A. thaliana root tip cells at a concentration of 10.0 μg mL^-1. Moreover, at a concentration of 0.1 mg mL^-1, compounds 3, 4, 6 and 7 inhibited the growth of probiotics and promoted the growth of pathogens of P. notoginseng. These results suggest that these isolated ursane-type triterpenoid acids and anthraquinone are potential allelochemicals that contribute to continuous-cropping obstacles of P. notoginseng.

Key words: Panax notoginseng, Continuous cropping obstacle, Allelochemical, Triterpenes, Anthraquinone

Yi-Jun Qiao, Cheng-Zhen Gu, Hong-Tao Zhu, Dong Wang, Meng-Yue Zhang, Yi-Xuan Zhang, Chong-Ren Yang, Ying-Jun Zhang. Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms[J]. Plant Diversity, 2020, 42(05): 323-333.

参考文献

Annamalai, T., Venkateswara, R.G., Mukhopadhyay, T., 2013. Pulmatin from the roots of Rumex acetosa. Der Pharm. Lett. 5, 116-119.
Bogatek, R., Gniazdowska, A., Zakrzewska, W., Oracz, K., Gawronski, S.W., 2006.Allelopathic effects of sunflower extracts on mustard seed germination and seedling growth. Biol. Plantarum 50, 156-158.
Chen, H.Y., Yu, X.M., Zhang, X.D., Yang, L., Huang, X., Zhang, J., Pritchard, H.W., Li, W.Q., 2017. Phospholipase Dα1-mediated phosphatidic acid change is a key determinant of desiccation-induced viability loss in seeds. Plant Cell Environ. 41, 50-63.
Chen, L.S., Lv, Y., Xu, S.W., Yi, Y., 2008. Study on the triterpene acids in fruit of Crataegus pinnatifida. Lishizhen Med. Mater. Med. Res. 19, 2909-2910.
Chen, Z.J., Wang, Y., Sun, Y.Q., Feng, G.Q., 2002. Present situation investigation of Panax notoginseng planting industry. J. Chin. Med. Mater. 25, 387-389.
Cho, I.H., Lee, H.J., Kim, Y.S., 2012. Differences in the volatile compositions of ginseng species (Panax sp.). J. Agric. Food Chem. 60, 7616-7622.
Diwakar, R., Shaikh, I., Dawda, H., Mukundan, U., 2016. Phytochemical evaluation and quantitative estimation of corosolic and ursolic acid from Psidium guajava.World J. Pharmaceut. Res. 5, 1318-1327.
Gao, L.L., Xu, X.D., Nan, H.J., Yang, J.S., Chen, S.L., 2011. Chemical constituents in Rheum tanguticum. Chin. Tradit. Herb. Drugs 42, 443-446.
Gu, C.Z., Lv, J.J., Zhang, X.X., Qiao, Y.J., Yan, H., Li, Y., Wang, D., Zhu, H.T., Luo, H.R., Yang, C.R., Xu, M., Zhang, Y.J., 2015. Triterpenoids with promoting effects on the differentiation of PC12 cells from the steamed roots of Panax notoginseng. J. Nat.Prod. 78, 1829-1840.
Gu, C.Z., Qiao, Y.J., Wang, D., Zhu, H.T., Yang, C.R., Xu, M., Zhang, Y.J., 2017. New triterpenoid saponins from the steaming treated roots of Panax notoginseng.Nat. Prod. Res. 32, 294-301.
Guan, H.L., Chen, Y.J., Liu, S.Q., Zhang, W.D., Xia, C.F., 2006. Study on the relationship between root rot in Panax notoginseng and soil microbes. J. South China Normal Univ. (Soc. Sci. Ed.) 5, 706-709.
Guan, H.L., Chen, Y.J., Zhu, H.C., Zhang, W.D., Yin, F., Yang, J.Z., Liu, S.Q., 2005.Characteristic study of rhizome-microorganism in soil of diseased Panax notoginseng. Agric. Tec. (Santiago) 25, 56-70.
Guan, H.L., Yang, J.Z., Chen, Y.J., Cui, X.M., Wang, Y., Zhang, Y.F., 2010. Change of rhizospheric microbe colony in cultivated soil and its correlation to root rot disease in Panax notoginseng. Soils 3, 378-384.
Hoagland, R.E., Williams, R.D., 2004. Bioassays-useful tools for the study of allelopathy. Libr. Work Study 39, 1-10.
Hu, Z.B., Vanderhaeghen, R., Cools, T., Wang, Y., Clercq, I.D., Leroux, O., Nguyen, L., Belt, K., Millar, A.H., Audenaert, D., Hilson, P., Small, I., Mouille, G., Vernhettes, S., Breusegem, F.V., Whelan, J., Höfte, H., Veyldera, L.D., 2016. Mitochondrial defects confer tolerance against cellulose deficiency. Plant Cell 28, 2276-2290.
Inderjit, 2006. Experimental complexities in evaluating the allelopathic activities in laboratory bioassays: a case study. Soil Biol. Biochem. 38, 256-262.
Jiang, Y.P., Li, H., Pan, X.Z., Sun, X.G., Tang, Q.F., Shao, M., 2013. Chemical constituents from the roots of Ilex pubescens. J. Chin. Med. Mater. 36, 1774-1778.
Ju, J.H., Zhou, L., Lin, G., Liu, D., Wang, L.W., Yang, J.S., 2003. Studies on constituents of triterpene acids from Eriobotrya japonica and their anti-inflammatory and antitussive effects. Chin. Pharmaceut. J. 38, 752-757.
Li, Y., Liu, S.L., Huang, X.F., Ding, W.L., 2009. Allelopathy of ginseng root exudates on pathogens of ginseng. Acta Ecol. Sin. 29, 161-168.
Liu, C.K., Deng, H.P., Yin, C., Lei, S.Y., 2010. Allelopathic effects of Chenopodium ambrosioides L. On seed germination and seedling growth in five crops. J. South China Normal Univ. (Soc. Sci. Ed.) 350, 152-155.
Liu, M.L., Luo, G.X., Wang, Y.Z., Xu, R., Wang, Y., He, W.F., Tan, J.L., Xing, M., Wu, J., 2017. Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing. Sci. Rep. 7, 436.
Miao, C.P., Mi, Q.L., Qiao, X.G., 2015. Rhizospheric fungi of Panax notoginseng: diversity and antagonism to host phytopathogens. J. Ginseng Res. 21, 127-134.
Ng, T.B., 2010. Pharmacological activity of sanchi ginseng (Panax notoginseng).J. Pharm. Pharmacol. 58, 1007-1019.
Niu, B.J., Ma, Z.K., Liao, Z.X., Ji, L.J., Sun, H.F., 2013. Chemical constituents from Dracocephalum heterophyllum. Chin. Tradit. Herb. Drugs 44, 147-152.
Passari, A.K., Mishra, V.K., Singh, G., Singh, P., Kumar, B., Gupta, V.K., Sarma, R.K., Saikia, R., Donovan, A.O., Sing, B.P., 2017. Author Correction: insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production. Sci. Rep. 7, 11809.
Qiao, Y.J., Shang, J.H., Wang, D., Zhu, H.T., Yang, C.R., Zhang, Y.J., 2018. Research of Panax spp. in Kunming Institute of botany. Nat. Prod. Bioprospect. 8, 245-263.
Romagni, J.G., 2004. Allelopathic effect of volatile cineoles on two weedy plant species. J. Chem. Ecol. 26, 303-313.
Shaw, P., Dolan, L., 2008. Chromatin and Arabidopsis root development. Semin. Cell Dev. Biol. 19, 580-585.
Sun, Y.Q., Chen, Z.J., Li, G.C., Yang, S.C., Wei, M.L., Cui, X.M., 2008a. Preliminary study of allelochemicals on propagation of Sanqi pathogen. Res. Pract. Chin. Med. 22, 19-21.
Sun, Y.Q., Wei, M.L., Chen, Z.J., Ke, J.H., 2008b. Preliminary study of allelochemicals influence on the seed germination of Panax Notoginseng (Burk.) F.H. Chen. Spec.Wild Econ. Anim. Plant Res. 30, 44-46.
Wang, C.Z., McEntee, E., Wicks, S., Wu, J.A., Yuan, C.S., 2006. Phytochemical and analytical studies of Panax notoginseng (Burk.) F.H. Chen. J. Nat. Med. 60, 97-106.
Wang, T., Guo, R.X., Zhou, G.H., Zhou, X.D., Kou, Z.Z., Sui, F., Li, C., Tang, L.Y., Wang, Z.J., 2016. Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: a review. J. Ethnopharmacol. 188, 234-258.
Wang, Y., Fan, C., Chen, Y.J., Liu, Y.Z., 2003. Main disease and prevention status of Panax notoginseng. Res. on Ginseng 15, 43-45.
Wu, X.P., Huang, X.Y., Zhang, X.P., Ma, G.X., Huang, Z., Yuan, J.Q., Xu, X.D., Zhong, X.M., 2014. Triterpenoid components from Rosa cymosa. Chin. Tradit.Herb. Drugs 45, 626-630.
Xiao, Y.H., Li, J., Liu, Z.X., Li, Y.J., Li, G., Long, H., 2013. Advances in studies on rhizospheric microorganism of medicinal plants. Chin. Tradit. Herb. Drugs 44, 497-504.
Xie, G.X., Qiu, Y.P., Qiu, M.F., Gao, X.F., Liu, Y.M., Jia, W., 2007. Analysis of dencichine in Panax notoginseng by gas chromatographyemass spectrometry with ethyl chloroformate derivatization. J. Pharmaceut. Biomed. Anal. 43, 920-925.
Xie, J., Wu, Y.Y., Zhang, T.Y., Zhang, M.Y., Zhu, W.W., Gullen, E.A., Wang, Z.J., Cheng, Y.C., Zhang, Y.X., 2017. New and bioactive natural products from an endophyte of Panax notoginseng. RSC Adv. 7, 38100-38109.
Xun, L.L., Zhao, H.G., Liang, Z.S., Wei, M.T., Liu, F.H., Han, R.L., 2013. Study of microecology in root rot and healthy Panax notoginseng soil. Acta Agric. Borealioccidentalis Sin. 22, 146-151.
Yang, M., Zhang, X.D., Xu, Y.G., Mei, X.Y., Jiang, B.B., Liao, J.J., Yin, Z.B., Zheng, J.F., Zhao, Z., Fan, L.M., He, X.H., Zhu, Y.Y., Zhu, S.S., 2015a. Autotoxic ginsenosides in the rhizosphere contribute to the replant failure of Panax notoginseng. PloS One 10, e0118555.
Yang, S.G., Li, C.L., Zhao, L.M., Gao, S.J., Lu, J.X., Zhao, M.L., Chen, C.Y., Liu, X.C., Luo, M., Cui, Y.H., Yang, C.W., Wu, K.Q., 2015b. The arabidopsis SWI2/SNF2 chromatin remodeling ATPase BRAHMA targets directly to PINs and is required for root stem cell niche maintenance. Plant Cell 27, 1670-1680.
You, P.J., Wang, W.Q., Zhang, Y., 2009a. Allelopathic effects of extracts from root zone soil of Panax Notoginseng on notoginseng's seedlings. Southwest China J.Agric. Sci. 2, 308-310.
You, P.J., Zhang, Y., Wang, W.Q., 2009b. Allelopathic effects of continuous cropping soil of Panax Notoginseng on seed and seedling of some vegetables. Mod. Chin.Med. 5, 12-13.
Zhang, J.J., Li, Y., Guo, J.P., Du, B., He, G.C., Zhang, Y.J., Chen, R.Z., Li, J.R., 2018a. Lipid profiles reveal different responses to brown planthopper infestation for pest susceptible and resistant rice plants. Metabolomics 14, 120.
Zhang, Y.T., Wu, W.H., Zhou, F.J., Duan, J.Y., Zhang, C.P., Yin, X.X., 2018b. Chemical constituents from Potentilla discolor Bge. Guangzhou Chem. Ind. 46, 72-74.
Zhang, Z.H., Tao, S., Ye, B.X., Peng, Z.Q., Yuan, J.P., 2004. Pollution sources and identification of hydrocarbons in soil and sediment using molecular markers.Chin. J. Soil Sci. 35, 793-798.
Zhang, Z.L., Wang, W.Q., Yang, J.Z., 2010. Effects of continuous Panax notoginseng cropping soil on P. notoginseng seed germination and seedling growth. Soils 6, 1009-1014.
Zhao, H.L., Wang, Q., Ruan, X., Pan, C.D., Jiang, D.A., 2010. Phenolics and plant allelopathy. Molecules 15, 8933-8952.
Zhou, J.M., Zhang, W.B., Yang, J.Z., Cui, X.M., Zeng, H.C., Zhu, L., 2012. Preliminary isolation and identification of allelopathy from the rhizosphere soil of Panax notoginseng (Buck) F. H. Chen. Chin. Med. J. Res. Prac. 26, 14-16.
Zhu, L., Ma, N., Cui, X.M., Zhu, Y., Zhou, J.M., 2014. Analysis of volatile components in planting soil and crop residues of Panax notoginseng. Chin. Med. J. Res. Prac. 28, 3-5.
Zhu, Y., Teng, Y., Zhang, M.Y., Ma, W.T., Liu, W.X., 2015. Pollutant accumulation and microbiological characteristics change in Notoginseng-planting soils. Soils 47, 121-127.

Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

Allelochemicals of Panax notoginseng and their effects on various plants and rhizosphere microorganisms

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