酶促和非酶促抗氧化系统在玉米胚脱水耐性获得中的作用

doi:10.3724 SP.J.1143.2009.08227

Plant Diversity ›› 2009, Vol. 31 ›› Issue (03): 253-259.DOI: 10.3724 SP.J.1143.2009.08227

酶促和非酶促抗氧化系统在玉米胚脱水耐性获得中的作用

罗银玲1 , 2 , 宋松泉3, 兰芹英1

1 中国科学院西双版纳热带植物园, 云南勐腊 666303; 2 思茅师范高等专科学校, 云南普洱 666500; 3 中国科学院植物研究所, 北京 100093

收稿日期:2008-12-22 出版日期:2009-06-25 发布日期:2009-06-25
通讯作者: 宋松泉

Possible Involvement of Enzymatic and Non-enzymatic Antioxidant System in Acquisition of Desiccation Tolerance of Maize Embryos

LUO Yin-Ling^{1 , 2}, SONG Song-Quan³, LAN Qin-Ying1

1 Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences , Mengla 666303 , China ; 2 Simao Teacher′s College, Puer 665000 , China ; 3 Institute of Botany , Chinese Academy of Sciences , Beijing 100093 , China

Received:2008-12-22 Online:2009-06-25 Published:2009-06-25
Contact: SONG Song-Quan

摘要/Abstract

摘要： 以发育中的玉米胚为材料, 研究了玉米胚脱水耐性的发育变化及其与抗氧化系统之间的关系。结果表明, 授粉后18 d 的胚获得萌发能力, 但不耐脱水; 授粉后36 d 的胚开始获得耐脱水能力, 并随着发育逐渐增加。随着发育, 胚的超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX) 、谷胱甘肽还原酶(GR) 和脱氢抗坏血酸还原酶(DHAR) 的活性逐渐降低, 过氧化氢酶(CAT) 活性逐渐增加。授粉后16～22 d 的玉米胚中检测不到抗坏血酸, 24 d 后胚中抗坏血酸的含量显著增加; 还原性谷胱甘肽含量在整个发育过程中逐渐增加。脱水胚的SOD、APX 和DHAR 的活性比对照( 未脱水) 胚低, 而GR 和CAT 活性在发育早期比对照胚低, 在发育中、后期高于对照胚。脱水胚的抗坏血酸和还原性谷胱甘肽含量明显低于对照胚。胚中丙二醛的含量随着发育逐渐下降, 脱水胚的丙二醛含量显著高于对照。这些结果说明CAT 活性和谷胱甘肽含量的增加以及脂质过氧化产物丙二醛含量的下降与玉米胚脱水耐性的获得密切相关。

关键词: 抗氧化系统, 脱水耐性, 脂质过氧化, 玉米胚, 种子发育

Abstract: Developing maize embryos were used to investigate the relationship between developmental changes in desiccation tolerance and antioxidant system . The results indicated that maize embryos acquired germinability at 18 days after pollination(DAP), but were desiccation-sensitive .Maize embryos started to acquire desiccation tolerance at 36 DAP, and the tolerance to dehydration gradually increased with development . With embryo development , the activities of superoxide dismutase (SOD) , ascorbate peroxidase (APX), glutathione reductase (GR) and dehydroascorbate reductase (DHAR) gradually
decreased , while catalase (CAT) activity significantly increased . The ascorbic acid (AsA) contents could not be detected in 16 - 22 DAP embryos, and notably increased in 24- 50 DAP embryos. Reduced glutathione (GSH) contents gradually increased during development of embryos . The activities of SOD, APX and DHAR in dehydrated embryos were lower than those in control ( non-dehydrated embryos), however , the CAT and GR activities were higher in control embryos thanin dehydrated samples during the early stage of development , and were lower during the middle and late stages . Contents of AsA and GSH of dehydrated embryos obviously decreased compared with non-dehydrated embryos. Malondialdehyde (MDA) contents of embryos gradually decreased with development , and those of dehydrated embryos were higher than control. These results showed that the increase in catalase activity and AsA and GSH contents, and decrease in MDA content were closely related to acquisition of desiccation tolerance of maize embryos .

Key words: Antioxidant system

中图分类号:

Q 945

罗银玲1 , 2 , 宋松泉3, 兰芹英1 . 酶促和非酶促抗氧化系统在玉米胚脱水耐性获得中的作用[J]. Plant Diversity, 2009, 31(03): 253-259.

LUO Yin-Ling^,, SONG Song-Quan, LAN Qin-Ying. Possible Involvement of Enzymatic and Non-enzymatic Antioxidant System in Acquisition of Desiccation Tolerance of Maize Embryos[J]. Plant Diversity, 2009, 31(03): 253-259.

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酶促和非酶促抗氧化系统在玉米胚脱水耐性获得中的作用

Possible Involvement of Enzymatic and Non-enzymatic Antioxidant System in Acquisition of Desiccation Tolerance of Maize Embryos

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