脱水速率对非洲柚种子脱水耐性的影响

doi:10.7677/ynzwyj201514041

摘要/Abstract

摘要：

通过脱水速率对不同发育阶段的非洲柚种子脱水耐性影响的研究，其结果表明：花后130d，190d，245d和275d的非洲柚种子在慢速脱水的条件下具有比快速脱水更强的脱水耐性，而且差异显著 (P < 005)。但对于花后155d和220d的非洲柚种子，快速脱水和慢速脱水条件下脱水耐性差别变小，未达到显著性水平；脱水速率对非洲柚种子脱水耐性的影响与种子的发育阶段有关。这样的结果与前人以正常性大豆种子和玉米胚为材料得到的结果相类似。研究认为，脱水速率对中间型非洲柚种子脱水耐性的影响，可能主要在于慢速脱水能够诱导某些与脱水耐性相关的蛋白的表达和积累。慢速脱水诱导脱水耐性必需以种子具有相应的遗传基础为前提，而且需要选择合适的时机。

关键词: 非洲柚, 脱水速率, 发育阶段, 脱水耐性, 中间型种子

Abstract:

The effects of drying rates on the desiccation tolerance of Citrus maxima ‘Feizhouyou’ seeds at different developmental stages were studied in this paper. For seeds harvested at 130 days after anthesis (DAA), 190 DAA, 245 DAA and 275 DAA, slowdried seeds had higher desiccation tolerance than those rapiddried, with difference at significant level (P < 005). However, such improvement was little for seeds harvested at 155 DAA and 220 DAA, indicating that effect of drying rate on desiccation tolerance depends on seed developmental stages. These results accorded with previous reports on orthodox soybean seeds and maize embrys. It was suggested that the effects of drying rate on desiccation tolerance of intermediate Citrus maxima ‘Feizhouyou’ seeds mainly resulted from expression and accumulation of some desiccationrelated proteins induced by slow drying. On the required genetic basis, desiccation tolerance in seeds can be induced only at suitable seed developmental stages.

Key words: Citrus maxima, Feizhouyou, Drying rates, Developmental stage, Desiccation tolerance, Intermediate seeds

中图分类号:

Q 945

薛鹏, 文彬. 脱水速率对非洲柚种子脱水耐性的影响[J]. Plant Diversity, 2015, 37(03): 293-300.

XUE Peng, WEN Bin. Effects of Drying Rates on the Desiccation Tolerance of Citrus maxima ‘Feizhouyou’ Seeds[J]. Plant Diversity, 2015, 37(03): 293-300.

参考文献

Berjak P, Pammenter NW, 1994. Recalcitrance is not an allornothing situation［J］. Seed Science Research, 4: 263—264
Berjak P, Pammenter NW, 1997. Progress in the understanding and manipulation of desiccationsensitive (recalcitrant) seeds［A］// Ellis RH, Black M, Murdoch AJ et al. eds., Basic and Applied Aspects of Seed Biology［M］. Dordrecht: Kluwer Academic Publishers, 689—703
Berjak P, Pammenter NW, 2001. Seed recalcitrancecurrent perspectives［J］. South African Journal of Botany, 67: 79—89
Berjak P, Vertucci CW, Pammenter NW, 1993. Effects of developmental status and dehydration rate on characteristics of water and desiccationsensitivity in recalcitrant seeds of Camellia sinensis［J］. Seed Science Research, 3: 155—156
Blackman SA, Obendorf RL, CarlLeopold A, 1992. Maturation proteins and sugars in desiccation tolerance of developing soybean seeds［J］. Plant Physiology, 100: 225—230
Bradford KJ, Chandler PM, 1992. Expression of dehydrinlike proteins in embryos and seedlings of Zizania palustris and Oryza sativa during dehydration［J］. Plant Physiology, 99: 488—494
Daws MI, Lydall E, CHmielarz P et al., 2004. Developmental heat sum influence recalcitrant seed traits in Aesculuc hippocastanum across Europe［J］. New Phytologist, 162: 157—166
Daws MI, Cleland H, Chmielarz P et al., 2006. Variable desiccation tolerance in Acer pseudoplantanus seeds in relation to developmental conditions: a case of phenotypic recalcitrance?［J］. Functional Plant Biology, 33: 59—66
Dickie JB, Pritchard HW, 2002. Systematic and evolutionary aspects of desiccation tolerance in seeds［A］// Black M, Pritchard HW eds., Desiccation and Plant Survival［M］. CABI Publishing, Wallingford, 239—259
Dussert S, Chabrillange N, Engelmann F et al., 1999. Quantitative estimation of seed desiccation sensitivity using a quantal response model: application to nine species of the genus Coffea L［J］. Seed Science Research, 9: 135—144
Eira MTS, Walters C, Caldas LS et al., 1999. Tolerance of Coffea spp. seeds to desiccation and low temperature［J］. Revista Brasileira de Fisiologia Vegetal, 11: 97—105
Ellis RH, Hong TD, Roberts EH, 1990. An intermediate category of seed storage behaviour? I. Coffer［J］. Journal of Experimental Botany, 41: 1167—1174
Ellis RH, Hong TD, Roberts EH, 1991. An intermediate category of seed storage behaviour? II. Effects of provenance, immaturity, and imbibition on desiccation tolerance in coffee［J］. Journal of Experimental Botany, 42: 653—657
Farrant JM, Berjak P, Pammenter NW, 1993. Studies on the development of the desiccationsensitive (recalcitrant) seeds of Avicennia marina (Forssk.) Vierh: the acquisition of germinability and response to storage and dehydration［J］. Annals of Botany, 71: 405—410
FinchSavage WE, Hendry GAF, Atherton NM, 1994. Free radical activity and loss of viability during drying of desiccationsensitive tree seeds［J］. Proceedings of the Royal Society of Edinburg, 102: 257—260
Fu JR (傅家瑞), Song SQ (宋松泉), 2001. Advances in study on desiccation tolerance of seeds［J］. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 9: 345—354
Fu JR, Yang XQ, Jiang XC et al., 1997. Heatstable proteins and desiccation tolerance in recalcitrant and orthodox seeds［A］// Ellis RH, Black M, Murdoch AJ et al. eds., Basic and Applies Aspects of Seed Biology［M］. Dordrecht: Kluwer Academic Publishers, 705—713
Galau GA, Bijaisoradat N, Hughes DW, 1987. Accumulation kinetics of cotton late embryogenesisabundant mRNAs and storage protein mRNAs: Coordinate regulation during embryogenesis and the role of abscisic acid［J］. Developmental Biology, 123: 198—212
Gee OH, Probert RJ, Coomber SA, 1994. Dehydrinlike proteins and desiccation tolerance in seeds［J］. Seed Science Research, 4: 135—142
Hay F, Probert R, Marro J et al., 2000. Towards the conservation of aquatic angiosperma: a review of seed storage behaviour［A］// Black M, Bradford KJ, VazquezRamos J eds., Seed Biology Advances and Applications［M］. Oxford. CABI Publishing, 161—177
Hendry GAF, FinchSavage WE, Thorpe PC et al., 1992. Free radical processes and loss of viability during desiccation in the recalcitrant species Quercus rober L［J］. New Phytologist, 122: 273—279
Hong TD, Ellis RH, 1995. Interspecific variation in seed storage behavior within two genera—Coffea and Citrus［J］. Seed Science and Technology, 23: 165—181
Hong TD, Ellis RH, 1996. A Protocol to Determine Seed Storage Behaviour［M］. IPGRI Technical Bulletin No1. Rome: International Plant Genetic Resources Institute
Hong TD, Linington SH, Ellis RH, 1998. Compendium of Informationon on Seed Storage Behavior［M］, Vols 1 and 2. Royal Botanic Gardens, Kew, UK, 901
Honjo H, Nakagawa Y, 1978. Suitable temperature and seed moisture content for maintaining the germinability of citrus seed for longstem storage［A］// Akihama T, Nakajima K eds., Long Term Preservation of Favourable Germplasm in Arboreal Crops［M］. Ministry of Agriculture and Forestry, Tokyo, Japan, 31—35
Hor YL, Kim YJ, Ugap A et al., 2005. Optimal hydration status for cryopreservation of intermediate oily seeds: Citrus as a case study［J］. Annals of Botany, 95: 1153—1161
Huang H, Song SQ , Wu XJ, 2009. Response of chinese wampee axes and maize embryos to dehydration at different rates［J］. Journal of Integrative Plant Biology, 51: 67—74
International Seed Testing Association, 2004. International Rules for Seed Testing［M］. Switzerland: The International Seed Testing Association, CH8046 Zurich
Kermode AR, 1997. Approaches to elucidate the basis of desiccationtolerance in seeds［J］. Seed Science Research, 7: 75—95
Liang YH, Sun WQ, 2000. Desiccation tolerance of recalcitrant Theobroma cacao embryonic axes: the optimal drying rate and its physiological basis［J］. Journal of Experimental Botany, 51: 1911—1919
Liang YH, Sun WQ, 2002. Rate of dehydration and cumulative desiccation stress interacted to modulate desiccation tolerance of recalcitrant cocoa and ginkgo embryonic tissues［J］. Plant Physiology, 128: 1323—1331
Malik SK, Chaudhury R, Pritchard HW, 2012. Longterm, large scale banking of citrus species embryos: comparisons between cryopreservation and other seed banking temperatures［J］. CryoLetters, 33: 453—464
Ouedraogo AS, Thomsen K, Engels JMM et al., 1999. Challenges and opportunities for enhanced use of recalcitrant and intermediate tropical forest tree seeds through improved handling and storage［A］// Marzalina M, Khoo KC, Tsan FY et al. eds., Seed Symposium 1998 ‘Recalcitrant Seeds’, 12-15 October 1998［M］. Kuala Lumpur: IUFRO, 227—234
Pammenter NW, Berjak P, 1999. A review of recalcitrant seed physiology in relation to desiccation tolerance mechanisms［J］. Seed Science Research, 9: 13—37
Pammenter NW, Berjak P, Walters C, 2000. The effects of drying rate on recalcitrant seeds: lethal water content, causes of damage, and quantification of recalcitrance［A］// Black M, Bradford KJ, VazquezRamos J eds., Seed Biology: Advances and Applications［M］. CABI Publishing, Oxon, 215—221
Pammenter NW, Greggains V, Kioko JI et al., 1998. Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis［J］. Seed Science Research, 8: 463—471
Pammenter NW, Vertucci CW, Berjak P, 1991. Homeohydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii［J］. Plant Physiology, 96: 1093—1098
Pritchard HW, Manger KR, 1998. A calorimetric perspective on desiccation stress during preservation procedures with recalcitrant seeds of Quercus robur L［J］. Cryoletters, 19: 23—30
Roberts EH, 1973. Predicting the storage life of seeds［J］. Seed Science and Technology, 1: 499—514
Samarah NH, Mullen RE, Alqudah AM, 2009. An index to quanitify seed moisture loss rate in relationship with seed desiccation tolerance in common vetch［J］. Seed Science and Technology, 37: 413—422
Smith MT, Berjak P, 1995. Deteriorative changes associated with the loss of viability of stored desiccationtolerance and desiccationsensitive seeds［A］// Kigel J, Galili G eds., Seed Development and Germination［M］. New York: Marcel Dekker Inc, 701—746
Thomann EB, Sollinger J, White C et al., 1992. Accumulation of group 3 late embryogenesis abundant proteins in Zea mays embryos［J］. Plant Physiology, 99: 607—614
Tompsett PB, 1982. The effect of desiccation on the longevity of seeds of Araucaria hunsteinii and Acunninghamii［J］. Annals of Botany, 50: 693—704
Tompsett PB, 1984. Desiccation and storage studies on Dipterocarpm seeds［J］. Annals of Applied Biology, 105: 581—586
Tompsett PB, Kemp R, 1996. Database of Tropical Ttree Seed Reseaseh (DABATTS) ［M］. Datatase Contents. Royal Botanic Gardens, Kew. UK, 263
Tweddle JC, Dickie JB, Baskin CC et al., 2003. Ecological aspects of seed desiccation sensitivity［J］. Journal of Ecology, 91: 294—304
Walters C, Farrant JM, Pammenter NW et al., 2002. Desiccation stress and damage［A］// Black M, Pritchard HW eds., Desiccation and Survival in Plants: Drying Without Dying［M］. CABI Publishing, Oxon, 263—291
Wen B, 2011. Cytological and physiological changes related to cryotolerance in recalcitrant Livistona chinensis embryos during seed development［J］. Protoplasma, 248: 483—491
Wen B, Cai CT, Wang RL et al., 2010. Critical moisture content windows differ for the cryopreservation of pomelo (Citrus grandis) seeds and embryonic axes［J］. CryoLetters, 31: 29—39
Wen B, Wang RL, Song SQ, 2009. Cytological and physiological changes related to cryotolerance in orthodox maize embryos during seed development［J］. Protoplasma, 236: 29—37
WesleySmith J, Pammenter NW, Berjak P et al., 2001. The effects of two drying rates on the desiccation tolerance of embryonic axes of recalcitrant jackfruit (Artocarpus heterophyllus Lamk.) seeds［J］. Annals of Botany, 88: 653—664
Yang XQ (杨晓泉), Jiang XC (姜孝成), Fu JR (傅家瑞), 1998. Heatstable proteins and acquisition of desiccation tolerance in peanut seeds［J］. Acta Botanica Sinica (植物学报), 40: 337—342
Zhang N, Wen B, Ji MY et al., 2014. Lowtemperature storage and cryopreservation of grapefruit (Citrus paradisi Macfad.) seeds［J］. Cryoletters, 35: 418—426
Zheng GH, Jing XM, 1998. Ultradry seed storage cuts cost of gene bank［J］. Nature, 393: 223—224植物分类与资源学报 2015, 37(3)：301~308

[1]	杨娅娟, 郭永杰, 秦少发, 胡枭剑. 云南九种樟科植物种子的萌发及脱水耐性[J]. Plant Diversity, 2015, 37(06): 813-820.
[2]	计明月, 张楠, 闫启, 文彬. 顽拗性竹柏种子的贮藏特性[J]. Plant Diversity, 2015, 37(01): 63-70.
[3]	张丽霞1, 兰芹英2, 李海涛1, 谭运洪2, 管燕红1, 李学兰1. 白木香种子脱水耐性的发育变化及贮藏特性[J]. Plant Diversity, 2011, 33(4): 458-464.
[4]	路信1,2, 罗银玲1,3, 王一帆1, 兰芹英1. 不同脱水速率对木奶果种子脱水敏感性及抗氧化酶活性的影响[J]. Plant Diversity, 2010, 32(4): 361-366.
[5]	罗银玲1 , 2 , 宋松泉3, 兰芹英1 . 酶促和非酶促抗氧化系统在玉米胚脱水耐性获得中的作用[J]. Plant Diversity, 2009, 31(03): 253-259.
[6]	宋松泉1 , Patricia BERJAK2 , Norman W . PAMMENTER2 . 豌豆种子吸胀过程中脱水耐性变化的时间模式[J]. Plant Diversity, 2009, 31(03): 239-246.
[7]	邓志军1 , 向振勇1 , 3 , 程红焱2 , 李燕军1 , 宋松泉1 , 2 . 麻疯树种子的发育、萌发和脱水耐性的初步研究[J]. Plant Diversity, 2008, 30(03): 355-359.
[8]	邵玉涛; 殷寿华; 兰芹英; 张晓燕. 假槟榔种子脱水耐性的发育变化[J]. Plant Diversity, 2006, 28(05): 515-522.
[9]	唐安军1 , 2 , 龙春林1 , 刀志灵1 , 宋松泉3 , 田美华2 , 3 . 棕榈种子萌发特性及其贮藏行为的研究[J]. Plant Diversity, 2005, 27(06): 657-662.
[10]	罗银玲，宋松泉. 玉米胚发育过程中脱水耐性的变化[J]. Plant Diversity, 2005, 27(03): 301-309.
[11]	钱春梅，类延宝，宋松泉. 预吸胀对绿豆种子脱水耐性的影响[J]. Plant Diversity, 2004, 26(06): 1-3.
[12]	宋松泉龙春林殷寿华兰芹英. 种子的脱水行为及其分子机制[J]. Plant Diversity, 2003, 25(04): 1-3.