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Plant Diversity ›› 2013, Vol. 35 ›› Issue (1): 41-47.doi: 10.7677/ynzwyj201312046

• 研究论文 • 上一篇    下一篇

拟南芥WRKY68转录调控因子的表达谱分析

 王学兰1, 林良斌1, 余迪求2   

  1. 1 云南农业大学农学与生物技术学院,云南 昆明650201;2 中国科学院西双版纳热带植物园,云南 昆明650223
  • 收稿日期:2012-04-04 出版日期:2013-01-25 发布日期:2012-05-25

Expression Profiles of Arabidopsis WRKY68 Transcription Factor

 WANG  Xue-Lan-1, LIN  Liang-Bin-1, YU  Di-Qiu-2   

  1. 1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;
    2 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China
  • Received:2012-04-04 Online:2013-01-25 Published:2012-05-25

摘要:

WRKY基因家族是主要存在于植物中的转录因子,拟南芥中至少有74个成员。根据锌指结构特征和WRKY结构域的数目,可以将WRKY转录因子分为三大类。拟南芥WRKY68属于第Ⅱ类WRKY蛋白。通过GUS染色和qRTPCR分析各组织部位的表达情况,发现WRKY68在根中的表达量是最高的,其次是幼嫩的叶片和老的荚果中。各种处理条件下的表达水平显示,IAA和高温处理后,WRKY68的表达明显上调,PstDC3000、JA、SA、NAA轻微诱导WRKY68的表达,而Botrytis、NaCl、甘露醇、PEG、脱水、ACC、ABA抑制WRKY68的表达,根据以上实验结果,我们推测WRKY68可能参与生长素和温度调控的植物形态建成及发育过程。

关键词: AtWRKY68转录因子, GUS染色, 生物逆境, 非生物逆境, 植物激素

Abstract:

The transcription factors WRKY protein superfamily are mainly presented in plants,which contains at least 74 members in Arabidopsis. It can be classified into three groups according to the number of WRKY domains and the features of their zincfingerlike motif. Arabidopsis WRKY68 is one of the group Ⅱ WRKY proteins. GUS staining and qRTPCR analysis showed that the expression of WRKY68 in roots was the highest in all tissues and the second was in old siliques and young leaves. The expression profiles indicated that the expression levels of WRKY68 were elevated by IAA and heat treatments,and slightly induced by PstDC3000, JA, SA, NAA while which were inhibited by other treatments including Botrytis, NaCl, mannitol, PEG, dehydration, ACC, ABA.These results suggested that WRKY68 might be involved in plant morphogenesis and development which is regulated by auxin and temperature.

Key words: AtWRKY68, GUS staining, Biotic stress, Abiotic stress, Plant hormones

中图分类号: 

  • Q 75