六个核基因片段在中国颗粒野生稻中的单核苷酸多态性及其在群体遗传学研究中的应用

doi:10.7677/ynzwyj201312151

摘要/Abstract

摘要：

为了筛选可用于亚洲栽培稻的重要野生近缘物种颗粒野生稻群体遗传结构研究的有效 SNPs标记，本研究共选取Osgstf3、OsNAC6、BADH、XCP2、EMF2和 ERCC4 6个重要水稻功能基因，检测了来自中国云南和海南12份颗粒野生稻代表样品中的单核苷酸多态性，结果发现Osgstf3、OsNAC6、BADH和XCP2的单核苷酸多态性接近于零，但在 EMF2和ERCC4内含子区中检测到较低的核苷酸变异。利用EMF2和ERCC4片段对来自云南的3个天然群体和来自海南的2个天然群体共52个个体进行了群体遗传学研究，发现该物种具有高水平的遗传分化和低水平的遗传变异的独特群体遗传结构；这种剧烈的遗传分化不仅来自云南和海南两个地区之间，而且还来自地区内居群间，说明该野生稻生长的热带、亚热带森林生境的破坏导致了碎裂的居群间基因流强烈受阻。

关键词: 核基因, 颗粒野生稻, 单核苷酸多态性, 遗传变异, 群体遗传结构

Abstract:

A total of six rice genes of functional importance, including Osgstf3, OsNAC6, BADH, XCP2, EMF2 and ERCC4, were selected to assess suitable SNPs for use in an analysis of population genetic structure of Oryza granulata, a precious wild relative of Asian cultivated rice. An initial survey of 12 representative samples of O.granulata from Yunnan and Hainan, China, resolved few SNPs in Osgstf3, OsNAC6, BADH and XCP2, while low levels of variation were recorded within introns of EMF2 and ERCC4. A subsequent SNP survey was conducted within the EMF2 and ERCC4 fragments across a sample of 52 plants collected from three and two natural populations in Yunnan and Hainan, respectively. This survey showed that O.granulata exhibits fairly high genetic divergence between populations despite low levels of nucleotide diversity. Genetic divergence between populations was evident both between and within geographical regions. These findings imply that recent habitat fragmentation in tropical and subtropical forests led to severe restrictions to gene flow, which enhanced divergence among populations.

Key words: Nuclear gene, Oryza granulata, Single nucleotide polymorphisms, Genetic variation, Population genetic structure

中图分类号:

Q 751

吴春燕1、2, 高立志1. 六个核基因片段在中国颗粒野生稻中的单核苷酸多态性及其在群体遗传学研究中的应用[J]. Plant Diversity, 2013, 35(5): 537-546.

WU Chun-Yan-, GAO Li-Zhi. SNP Analysis of Six Nuclear Gene Fragments for Studying Population Genetics of Oryza granulata from China[J]. Plant Diversity, 2013, 35(5): 537-546.

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