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Plant Diversity ›› 2014, Vol. 36 ›› Issue (02): 233-236.DOI: 10.7677/ynzwyj201413084

• 研究论文 • 上一篇    下一篇

4种人参属植物基因组大小的测定

 潘跃芝1、2, 张亦弛3、4, 龚洵2, 李富生1   

  1. 1 云南农业大学农学与生物技术学院,云南 昆明650201;2 中国科学院昆明植物研究所资源植物
    与生物技术重点实验室,云南 昆明650201;3 中国科学院昆明植物研究所中国西南野生
    生物种质资源库,云南 昆明650201;4 中国科学院大学,北京100049
  • 收稿日期:2013-04-09 出版日期:2014-03-25 发布日期:2013-07-10

Estimation of Genome Size of Four Panax Species by Flow Cytometry*

 PAN  Ye-Zhi-1、2, ZHANG  Yi-Chi-3、4, GONG  Xun-2, LI  Fu-Sheng-1   

  1. 1 Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China; 2 Key Laboratory of
    Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    3 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 4 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-04-09 Online:2014-03-25 Published:2013-07-10

摘要:

人参属(Panax)植物是重要的药用植物资源。以水稻(Oryza sativa)为内标,利用流式细胞术测定了三七(Panax notoginseng)、屏边三七(Panax stipuleanatus)、越南三七(Panax vietnamensis)及假人参(Panax pseudoginseng)4种人参属植物的基因组大小。结果表明,4种植物的基因组大小各不相同,其中,三七的最大,而屏边三七的最小。本组数据将为该属植物的基因组学研究以及种群进化研究提供基础数据参考。

关键词: C-值, 流式细胞术, 人参属

Abstract:

Ginseng genus Panax, a member of family Araliaceae, consists of about 11 species in total. Most species of this genus are applied as tonic and medicinal plant resources. In this article, the genome size (CValue) of four ginseng species was estimated by flow cytometry using Oryza sativa as the internal reference. The results revealed that the CValues of four species are different from each other, and the CValue of Pnotoginseng is the largest, while the CValue of Pstipuleanatus is the smallest one.

Key words: Panax, C-Value, Flow cytometry (FCM)

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