冬油菜和春油菜在冷驯过程中膜脂分子的变化

doi:10.3724/SP.J.1143.2010.10038

Plant Diversity ›› 2010, Vol. 32 ›› Issue (4): 347-354.DOI: 10.3724/SP.J.1143.2010.10038

冬油菜和春油菜在冷驯过程中膜脂分子的变化

李利霞1,2 ，李唯奇1

1 中国科学院昆明植物研究所，云南昆明650204；2 中国科学院研究生院，北京100049

收稿日期:2010-02-24 出版日期:2010-08-25 发布日期:2010-04-07
基金资助:
国家自然科学基金NSFC (30670474；30870571)

Profiling the Changes of Molecular Species in Membrane Lipids during Coldacclimation in Winter and Spring Cultivars of Rapeseed (Brassica napus)

LI Li-Xia-1,2, LI Wei-Ai-1

1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049,China

Received:2010-02-24 Online:2010-08-25 Published:2010-04-07

摘要/Abstract

摘要：

研究了冬油菜和春油菜两种油菜品种在4℃冷驯3天后11类151种膜脂分子的变化，计算了其中膜脂的相对含量、双键指数和碳链长度值。结果发现油菜膜脂的膜脂分子组成模式及其冷驯诱导的膜脂分子组成变化与前期实验中的拟南芥情况非常相似，冷驯3天诱导的膜脂分子组成变化较小，但是膜脂的双键指数略有升高，春油菜双键指数和冷驯积累的脯氨酸含量都比冬油菜多。上述结果说明，油菜的冷驯需要更多的时间，春油菜对冷驯比较敏感。

关键词: 油菜, 冷驯, 抗低温能力, 膜脂分子, 双键指数

Abstract:

The changes of molecular species in membrane lipids during coldacclimation (CA) in winter and spring cultivars of rapeseed (Brassica napus) were profiled with ESIMS/MS based lipidomics. The membrane fluidity, the critical properties for plants to tolerate freeing injuring, was examined with double bond index (DBI) and carbon number of the fatty acid of the glycrolipid. The results indicated that the molecular composition of membrane lipids in the two cultivars under both normal growth condition and CA were similar to that of Arabidopsis reported previously and that DBI in spring cultivar accumulation subtly more than that in winter cultivar. The results suggested that CA of rapeseeds needs more than 3 days and that spring cultivar were more sensitive to CA, which was confirmed by the CAinduced proline accumulation.

Key words: Brassica napus, Coldacclimation, Freezing tolerance, Membrane lipids, Lipid molecular species, Double band index

中图分类号:

Q 945

李利霞1,2, 李唯奇1. 冬油菜和春油菜在冷驯过程中膜脂分子的变化[J]. Plant Diversity, 2010, 32(4): 347-354.

LI Li-Xia-, LI Wei-Ai. Profiling the Changes of Molecular Species in Membrane Lipids during Coldacclimation in Winter and Spring Cultivars of Rapeseed (Brassica napus)[J]. Plant Diversity, 2010, 32(4): 347-354.

参考文献

Bakht J, Bano A, Dominy P, 2006. The role of abscisic acid and low temperature in chickpea (Cicer arietinum) cold tolerance. II. Effects on plasma membrane structure and function \[J\]. Journal of Experimental Botany, 57 (14): 3707—3715
Boyer JS, 1982. Plant productivity and environment \[J\]. Science, 218: 443—448Buchanan B, Gruissem W, Jones R, 2002. Biochemistry & Molecular Biology of Plants \[M\]. Hoboken: John Wiley & Sons
Falcone DL, Ogas JP, Somerville CR, 2004. Regulation of membrane fatty acid composition by temperature in mutants of Arabidopsis with alterations in membrane lipid composition \[J\]. BMC Plant Biology, 4: 17Levitt J, 1980. Responses of Plants to Environmental Stresses \[M\]. 2d. New York: Academic Press, 254—259Li WQ, Li MY, Zhang WH et al, 2004. The plasma membranebound phospholipase D delta enhances freezing tolerance in Arabidopsis thaliana \[J\]. Nature Biotechnology, 22 (4): 427—433Li WQ, Wang RP, Li MY et al, 2008. Differential degradation of extraplastidic and plastidic lipids during freezing and postfreezing recovery in Arabidopsis thaliana \[J\]. The Journal of Biological Chemistry, 283 (1): 461—468Murakami Y, Tsuyama M, Kobayashi Y et al, 2000. Trienoic fatty acids and plant tolerance of high temperature \[J\]. Science, 287: 476—479Sakai A, Larcher W, 1987. Frost Survival of Plants: Responses and Adaptation to Freezing Stress \[M\]. Berlin; New York: SpringerVerlag, 41—47Thomashow MF, 1999. Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms \[J\]. Annual Review of Plant Physiology, 50: 571—599Sangwan V, Foulds I, Singh J et al, 2001. Coldactivation of Brassica napus BN115 promoter is mediated by structural changes in membranes and cytoskeleton, and requires Ca2+ influx \[J\]. The Plant Journal, 27 (1): 1—12Verkleij AJ, De Maagd R, LeunissenBijvelt J et al, 1982. Divalent cations and chlorpromazine can induce nonbilayer structures in phosphatidic acidcontaining model membranes \[J\]. Biochimca et Biophysica Acta, 684 (2): 255—262Welti R, Li WQ, Li MY et al, 2002. Profiling membrane lipids in plant stress responses. Role of phospholipase D α in freezinginduced lipid changes in Arabidopsis \[J\]. The Journal of Biological Chemistry, 277: 31994—32002

[1]	禹晓梅, 李唯奇. 大豆种子吸胀冷害抗性与其质体膜脂不饱和度正相关[J]. Plant Diversity, 2014, 36(02): 187-196.
[2]	李艳1、2，田波1、3，李唯奇1. 磷脂酶Dδ缺失加剧UV-B诱导的膜伤害[J]. Plant Diversity, 2011, 33(3): 299-305.
[3]	杨慧1、2 , 高波3, 李唯奇1. 金属离子胁迫产生植物膜脂过氧化的能力与离子的极化能力正相关[J]. Plant Diversity, 2011, 33(3): 293-298.
[4]	杨世雄，周善滋. 油菜花丝枯萎机理的探讨[J]. Plant Diversity, 2002, 24(03): 1-3.
[5]	陈善娜;刘继梅;游慧灵;朱红俊;秦志宝;洪国民；沈云光. 抗寒剂和高油菜素内酯对高原水稻抗冷性的影响[J]. Plant Diversity, 1997, 19(02): 1-3.
[6]	罗鸿源;高翔;彭志良;胡仲梅. 大白菜显性核不育基因向白菜型油菜上转育的遗传研究[J]. Plant Diversity, 1997, 19(01): 1-3.
[7]	韦仲新. 芥蓝与芸苔属几个常见种花粉形态的比较[J]. Plant Diversity, 1991, 13(03): 1-3.

冬油菜和春油菜在冷驯过程中膜脂分子的变化

Profiling the Changes of Molecular Species in Membrane Lipids during Coldacclimation in Winter and Spring Cultivars of Rapeseed (Brassica napus)

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价