我国西南元江大理茶的挥发性成分及其抗氧化活性

doi:10.3724/SP.J.1143.2012.12008

Plant Diversity ›› 2012, Vol. 34 ›› Issue (4): 409-.DOI: 10.3724/SP.J.1143.2012.12008

我国西南元江大理茶的挥发性成分及其抗氧化活性

朱利芳1、2, 董鸿竹1, 杨世雄3, 朱宏涛1, 许敏1, 曾淑芬4, 杨崇仁1、4, 张颖君1

1 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室，云南昆明650201；
2 中国科学院研究生院，北京100049；3 中国科学院昆明植物研究所生物多样性与生物地理学重点
实验室，云南昆明650201；4 云南维和药业股份有限公司技术中心，云南玉溪653101

收稿日期:2012-01-12 出版日期:2012-08-25 发布日期:2012-05-24
基金资助:
国家自然科学基金面上项目——茶组植物的化学成分及品质综合评价研究 (NSFC 30970287) 以及云南省林业厅项目——云南古茶的化学品质综合评价

Chemical Compositions and Antioxidant Activity of Essential Oil from Green Tea Produced from Camellia taliensis (Theaceae) in Yuanjiang, Southwestern China

ZHU Li-Fang-1、2, DONG Hong-Zhu-1, YANG Shi-Xiong-3, SHU Hong-Tao-1, XU Min-1, ZENG Shu-Fen-4, YANG Chong-Ren-1、4, ZHANG Ying-Jun-1

1 State Key Laboratory of Phytochemistry and Plant Resources of West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China;
3 Key Lab of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming
650201, China; 4 Biotech Center of Weihe Pharmaceutical Co., Ltd., Yuxi 653101, China

Received:2012-01-12 Online:2012-08-25 Published:2012-05-24

摘要/Abstract

摘要：

大理茶（Camellia taliensis）为山茶科山茶属茶组植物，主要分布于云南横断山脉澜沧江至伊洛瓦底江流域，即从云南的西部及西南部至缅甸北部。在其分布区，大理茶亦被称为野生大茶树，常用于加工制作茶叶。采用水蒸气蒸馏法、GC及GC/MS联用技术，首次对大理茶的鲜幼叶和鲜幼叶及老叶分别制成的绿茶中的挥发性成分进行提取和分析，共鉴定出91个化合物。研究结果表明，大理茶鲜幼叶的主要香气成分为棕榈酸(30.52%)，亚油酸(19.82%)，植醇(8.75%)和亚麻酸乙酯(2.54%)等有机酸及其酯和二萜类，而制成绿茶后，其主要香气成分则为芳樟醇(28.43%)，脱氢芳樟醇(1.13%)，α松油醇(11.68%)，橙花醇(4.92%)和香叶醇(12.34%)等单萜醇类成分。从大理茶鲜叶到由其制成的绿茶，香气成分发生了较大变化，形成了28种原鲜叶中未检测到的香气成分，其中，(Z, Z, Z)9, 12, 15十八烷三烯1醇的含量分别达到1.21% （幼叶绿茶）和11.2%（老叶绿茶），是大理茶制作的绿茶的特征香气成分。DPPH和ABTS+自由基清除实验结果显示大理茶鲜叶及其制成的绿茶的挥发性成分均具有一定的抗氧化活性，但均弱于茶多酚的抗氧化活性。

关键词: 大理茶, 鲜叶, 绿茶, 挥发性成分, 水蒸汽蒸馏法, 抗氧化活性

Abstract:

Camellia taliensis belonging to Camellia sect. Thea (Theaceae) is distributed from the western and southwestern areas of Yunnan Province, China to the north of Myanmar. Known as the “wild” tea plant, it has been commonly used for making tea by the local people of its growing area. It is the first investigation of the volatile constituents of the fresh tender leaves of C.taliensis and green teas produced from its tender and older leaves. The volatile constituents were obtained by hydrodistillation and analyzed by GC and GCMS. Ninetyone compounds were identified. The results showed that the main compositions of volatile oil of the fresh tender leaves were hexadecanoic acid (30.52%), linoleic acid (19.82%), phytol (8.75%), and geraniol (2.54%), while monoterpenoids (58.51%) composing of linalool (28.43%), hotrienol (1.13%), αterpineol (11.68%), nerol (4.92%) and geraniol (12.34%) were the major volatile components of its green tea product. From the fresh leaves to the green tea products, 28 aroma components were formed. Among then, the content of (Z, Z, Z)9, 12, 15octadecatrien1ol (peak 77) was up to 1.21% (from tender leaves) and 11.2% (from older leaves), respectively. The DPPH and ABTS+ radical scavenging assays demonstrated a moderate activity of essential oil from the three essential oils of C.taliensis.

Key words: Camellia taliensis, Fresh tender leaves, Green tea products, Essential oil, Hydrodistillation, Antioxidant assays

中图分类号:

Q 946

朱利芳1、2, 董鸿竹1, 杨世雄3, 朱宏涛1, 许敏1, 曾淑芬4, 杨崇仁1、4, 张颖君1. 我国西南元江大理茶的挥发性成分及其抗氧化活性[J]. Plant Diversity, 2012, 34(4): 409-.

ZHU Li-Fang-, DONG Hong-Zhu-, YANG Shi-Xiong-, SHU Hong-Tao-, XU Min-, ZENG Shu-Fen-, YANG Chong-Ren-, ZHANG Ying-Jun. Chemical Compositions and Antioxidant Activity of Essential Oil from Green Tea Produced from Camellia taliensis (Theaceae) in Yuanjiang, Southwestern China[J]. Plant Diversity, 2012, 34(4): 409-.

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我国西南元江大理茶的挥发性成分及其抗氧化活性

Chemical Compositions and Antioxidant Activity of Essential Oil from Green Tea Produced from Camellia taliensis (Theaceae) in Yuanjiang, Southwestern China

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