三种兜兰属植物种子发育过程及其与无菌萌发的关系

doi:10.7677/ynzwyj201312049

Plant Diversity ›› 2013, Vol. 35 ›› Issue (1): 33-40.DOI: 10.7677/ynzwyj201312049

三种兜兰属植物种子发育过程及其与无菌萌发的关系

张娟娟1、2, 严宁1, 胡虹1

1 中国科学院昆明植物研究所资源植物与生物技术所重点实验室，云南昆明650201；
2 中国科学院大学，北京100049

收稿日期:2012-04-09 出版日期:2013-01-25 发布日期:2012-08-17
基金资助:
云南省自然科学基金 (2009CD116)，中国科学院西部之光人才培养计划

The Seed Development of Three Paphiopedilum Species in Relation to Asymbiotic Germination

ZHANG Juan-Juan-1、2, YAN Ning-1, HU Hong-1

1 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-04-09 Online:2013-01-25 Published:2012-08-17

摘要/Abstract

摘要：

某些兰科植物的种子成熟前，在无菌萌发过程中有较高的萌发率，而成熟后萌发率急剧下降。本研究针对这一现象，对三种兜兰属植物种子的发育过程进行了解剖学观察，并对授粉后不同时期的种子进行无菌播种，80d后统计其萌发率，以探究种子发育过程中的解剖学特征与萌发率之间的关系。研究结果表明种胚刚发育到椭球形胚的时候，胚柄尚存，种皮细胞尚未皱缩，此时三种兜兰的萌发率都能达到较高水平，表明这是适合兜兰属植物萌发的最佳时期；此后，种皮开始皱缩，木质素类物质积累导致种皮透水性下降，推测这可能是导致其后期萌发率下降的主要因素之一。

关键词: 兜兰属, 种子发育, 无菌萌发, 解剖学

Abstract:

There is an optimum time for the asymbiotic germination of some species in Orchidaceae. A better understanding of the seed development would provide information for the germination events. The relationship between asymbiotic germination rate and the anatomical features in seed development of three Paphiopedilum species was studied. The highest frequency of seed germination was obtained when the embryo began to take an ellipsoidal shape, yet the seed coat had not dehydrated, and the suspensor cells were still present. After that, the parenchyma cell of the seed coat began to dehydrate and lignin substances accumulated, therefore lead to the down shift of the permeability of the seed coat, which maybe the main obstacles for the germination of the mature seeds of the Paphiopedilum species.

Key words: Paphiopedilum, Seed development, Anatomy, Asymbiotic germination

中图分类号:

Q 945

张娟娟1、2, 严宁1, 胡虹1. 三种兜兰属植物种子发育过程及其与无菌萌发的关系[J]. Plant Diversity, 2013, 35(1): 33-40.

ZHANG Juan-Juan-, YAN Ning-, HU Hong. The Seed Development of Three Paphiopedilum Species in Relation to Asymbiotic Germination[J]. Plant Diversity, 2013, 35(1): 33-40.

参考文献

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三种兜兰属植物种子发育过程及其与无菌萌发的关系

The Seed Development of Three Paphiopedilum Species in Relation to Asymbiotic Germination

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