马尾松中的抗氧化成分

doi:10.7677/ynzwyj201312104

Plant Diversity ›› 2013, Vol. 35 ›› Issue (2): 209-215.DOI: 10.7677/ynzwyj201312104

马尾松中的抗氧化成分

张玉梅1, 龚庆芳1、2, 杨佳倩1、3, 曾广智1, 谭宁华1

1 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室，云南昆明650201;
2 湖南师范大学，湖南长沙410081; 3 海南大学，海南海口570228

收稿日期:2012-08-16 出版日期:2013-03-25 发布日期:2012-11-06
基金资助:
The National Natural Science Foundation of China (20972168, 30725048), the National New Drug Innovation Great Project

of China (2011ZX09307-002-02), the Natural Science Foundation of Yunnan Province (2010CI048), and the Found of SKL-PPRWC, KIB, CAS (52P2010-ZZ07)

Antioxidant Constituents from Pinus massoniana (Pinaceae)

ZHANG Yu-Mei-1, GONG Qing-Fang-1、2, YANG Jia-Qian-1、3, CENG Guang-Zhi-1, TAN Ning-Hua-1

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany,
Chinese Academy of Sciences, Kunming 650201, China; 2 Hunan Normal University,
Changsha 410081, China; 3 Hainan University, Haikou 570228, China

Received:2012-08-16 Online:2013-03-25 Published:2012-11-06
Supported by:
The National Natural Science Foundation of China (20972168, 30725048), the National New Drug Innovation Great Project

of China (2011ZX09307-002-02), the Natural Science Foundation of Yunnan Province (2010CI048), and the Found of SKL-PPRWC, KIB, CAS (52P2010-ZZ07)

摘要/Abstract

摘要：

从马尾松(Pinus massoniana)枝条中分离得到21个已知化合物，经波谱学方法将其结构鉴定为7, 13, 15-abietatrien-18-oic acid (1), 12-methoxy-7, 13-abietadien-18-oic acid (2), 15-methoxy-7, 13-abietadien-18-oic acid methyl ester (3), 12-hydroxydehydroabietic acid (4), dehydroabietic acid (5), 15-hydroxydehydroabietic acid methyl ester (6), 3β-methoxyserrat-14-en-21-one (7), 3β-methoxy-30-hydroxyserrat-14-en-21-one (8), β-sitosterol (9), (±)-pinoresinol (10), (±)-matairesinol (11), (-)-nortrachelogenin (12), balanophonin (13), cedrusin (14), pinocembin (15), 5-methoxypinosylvin (16), pinosylvin (17), 1, 2-bis-(4-hydroxy-3-methoxyphenyl)-propane-1, 3-diol (18), cleistophostaudin (19), lignoceryl ferulate (20) 和coniferaldehyde (21)。其中化合物12, 14, 18和21 在DPPH抗氧化模型上显示一定的抗氧化活性，其EC50值分别为11.36, 6.76, 14.07和13.33μg·mL-1。化合物2~4, 6~8, 10, 11, 13, 15~21均是从该植物中首次分离得到。

关键词: 马尾松, 化学成分抗氧化活性

Abstract:

Twenty one known compounds were isolated from the twigs of Pinus massoniana. Their structures were elucidated as 7, 13, 15-abietatrien-18-oic acid (1), 12-methoxy-7, 13-abietadien-18-oic acid (2), 15-methoxy-7, 13-abietadien-18-oic acid methyl ester (3), 12-hydroxydehydroabietic acid (4), dehydroabietic acid (5), 15-hydroxydehydroabietic acid methyl ester (6), 3β-methoxyserrat-14-en-21-one (7), 3β-methoxy-30-hydroxyserrat-14-en-21-one (8), β-sitosterol (9), (±)-pinoresinol (10), (±)-matairesinol (11), (-)-nortrachelogenin (12), balanophonin (13), cedrusin (14), pinocembin (15), 5-methoxypinosylvin (16), pinos、lvin (17), 1, 2-bis-(4-hydroxy-3-methoxyphenyl)-propane-1, 3-diol (18), cleistophostaudin (19), lignoceryl ferulate (20) and coniferaldehyde (21). Compounds 12, 14, 18 and 21 showed antioxidant activities on DPPH assay with EC50 values of 11.36, 6.76, 14.07, and 13.33μg·mL-1, respectively. Compounds 2-4, 6-8, 10, 11, 13 and 15-21 were isolated from this plant for the first time.
Key words: Pinus massoniana; Chemical constituents; Antioxidant activityPinus massoniana Lamb. belongs to the family of Pinaceae and is widely distributed in China. Its pollen and nodular branch are used as two traditional Chinese medicines, which can be used to treat traumatic hemorrhage, eczema, sprain and strain (Chinese Pharmacopoeia Commission, 2010). Previous investigations on P.massoniana led to the isolation of a series of lignans, flavones and diterpenoids (Feng et al., 2004; Bi et al., 2002; Shen and Theander, 1985; Wang et al., 2008; Yang et al., 2010), and the discovery of some antitumor diterpenes (Tanaka et al., 2008) and some antioxidant extracts (Pan et al., 2009). For the recent researches revealed that the occurrence of some chronic diseases such as cancer, cardiovascular disease and diabetes were main caused by oxidative stress, therefore antioxidant compounds might provide us some different insights for treating these severe diseases. In this paper, we report the isolation and structural elucidation of 21 known compounds from its twigs with the antioxidant activities.

Key words: Pinus massoniana, Chemical constituents, Antioxidant activity

中图分类号:

Q 946

张玉梅1, 龚庆芳1、2, 杨佳倩1、3, 曾广智1, 谭宁华1. 马尾松中的抗氧化成分[J]. Plant Diversity, 2013, 35(2): 209-215.

ZHANG Yu-Mei-, GONG Qing-Fang-, YANG Jia-Qian-, CENG Guang-Zhi-, TAN Ning-Hua. Antioxidant Constituents from Pinus massoniana (Pinaceae)[J]. Plant Diversity, 2013, 35(2): 209-215.

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马尾松中的抗氧化成分

Antioxidant Constituents from Pinus massoniana (Pinaceae)

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