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Plant Diversity ›› 2013, Vol. 35 ›› Issue (3): 348-354.DOI: 10.7677/ynzwyj201313037

• 研究论文 • 上一篇    下一篇

叶表皮蜡质纹饰特征的种内变异——以青藏高原地区的紫花针茅和小叶棘豆为例

 王东超1、2, 吴杰3、5, 杨永红1, 陈家辉2、3、4, 杨永平3、4   

  1. 1 云南农业大学云南省植物病理重点实验室,云南 昆明650201;2 中国科学院昆明植物研究所生物多样性与生物地
    理学重点实验室,云南 昆明650201;3 中国科学院昆明植物研究所西南野生生物种质资源库,云南 昆明650201;
    4 中国科学院昆明植物研究所青藏高原研究所昆明部,云南 昆明650201;5 中国科学院大学,北京100049
  • 收稿日期:2013-03-15 出版日期:2013-05-25 发布日期:2013-04-15
  • 基金资助:

    科技部科技基础性工作专项重点项目 (2007FY110100);国家自然科学基金 (30900085);云南省自然科学基金 (2010CD109);国家重点基础研究发展计划 (2010CB951704)

Intraspecific Variation of Leaf Epidermal Cuticle Waxes under Scanning Electronic Microscope:Stipa purpurea and Oxytropis microphylla from the QinghaiTibet Plateau

WANG  Dong-Chao-1、2, WU  Jie-3、5, YANG  Yong-Hong-1, CHEN  Jia-Hui-2、3、4, YANG  Yong-Ping-3、4   

  1. 1 Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China; 2 Key
    Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming  650201, China;
    3 Plant Germplasm and Genomics Center, China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 4 Institute of Tibetan Plateau Research at Kunming, Chinese Academy of Sciences,
    Kunming  650201, China; 5 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-03-15 Online:2013-05-25 Published:2013-04-15
  • Supported by:

    科技部科技基础性工作专项重点项目 (2007FY110100);国家自然科学基金 (30900085);云南省自然科学基金 (2010CD109);国家重点基础研究发展计划 (2010CB951704)

摘要:

叶表皮在扫描电镜下的微形态特征,特别是蜡质纹饰的微形态特征,被认为具有重要的分类学和系统学价值。既往的叶表皮微形态特征的研究主要集中于种或种以上分类等级,而对种内微形态特征变异的研究较少。本研究以紫花针茅和小叶棘豆为研究对象,各选取29份及15份来自青藏高原不同地区的实验材料,在扫描电镜下观察叶表皮蜡质纹饰微形态特征。结果表明:两种植物的蜡质纹饰微形态特征在种内都存在较大的变异,推测该特征并不受单一因素的选择作用,生境的异质性导致蜡质纹饰的多态性。本研究建议在使用叶表皮微形态特征开展分类学和系统学研究时,应加大取样量并涵盖尽可能多的生境类型,才有可能阐明一个物种叶表皮微形态特征的变异范围。

关键词: 青藏高原, 叶表皮, 蜡质纹饰, 种内变异, 紫花针茅, 小叶棘豆

Abstract:

Leaf epidermal microfeatures under Scanning Electronic Microscope (SEM), especially microfeatures of cuticle waxes, are considered to be a valuable character in plant taxonomy and systematics. Previously studies of leaf epidermal microfeatures mainly focused on levels of species or above species, whereas intraspecific study was rare. In order to explore intraspecific variation of leaf epidermal microfeatures, we selected multiple materials of Stipa purpurea and Oxytropis microphylla (29 and 15 samples, respectively) to observe the microfeatures of leaf cuticle waxes under SEM. The results revealed that these two species bearing high intraspecific variations of microfeatures of leaf cuticle waxes, and these variations lack of a strong correlation with any single biotic or abiotic factors, in contrast, it is affected by combined effects of multiple factors. Therefore, the use of microfeature of leaf cuticle waxes in taxonomical and systematic study will depend, to great extent, upon the detection of microfeature variation range, by extensive sampling along the whole distribution area.

Key words: Qinghai-Tibet Plateau, Leaf epidermis, Cuticle waxes, Intraspecific variation, Stipa purpurea, Oxytropis microphylla

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