三种甘草属植物的种间杂交亲和性及杂交种子活力

doi:10.7677/ynzwyj201413203

Plant Diversity ›› 2014, Vol. 36 ›› Issue (03): 342-348.DOI: 10.7677/ynzwyj201413203

三种甘草属植物的种间杂交亲和性及杂交种子活力

谢良碧1, 陆嘉惠1、2, 李晓岚1, 张宇1, 魏涛1, 李学禹2

1 石河子大学生命科学学院，新疆石河子832003；2 石河子大学甘草研究所，新疆石河子832003

收稿日期:2013-10-03 出版日期:2014-05-25 发布日期:2013-12-20
基金资助:
国家自然科学基金 (31260042)；国家大学生创新创业训练计划项目 (201310759019)

The Cross Compatibility and Hybrid Seed Vigor among Three Glycyrrhiza Species

XIE Liang-Bi-1, LU Jia-Hui-1、2, LI Xiao-Lan-1, ZHANG Yu-1, WEI Tao-1, LI Xue-Yu-2

1 College of Life Sciences of Shihezi University, Shihezi 832003, China; 2 Institute of Licorice
in Shihezi University, Shihezi 832003, China

Received:2013-10-03 Online:2014-05-25 Published:2013-12-20
Supported by:
国家自然科学基金 (31260042)；国家大学生创新创业训练计划项目 (201310759019)

摘要/Abstract

摘要：

通过新疆分布的3种甘草属植物——乌拉尔甘草(Glycyrrhiza uralensis)、光果甘草（Gglabra）和黄甘草（Geurycarpa）的人工杂交试验，比较了杂交结果率、杂交种子的萌发参数、幼苗成活率等指标，并对杂交授粉后花粉管萌发、生长过程进行荧光观察，旨在探讨3种甘草属植物的种间杂交亲和性及杂交后代种子活力。结果表明：（1）种间杂交结果率最高为9667%，最低为5562%，说明3种甘草种间杂交配子亲和性较高；（2）荧光显微观察表明，各杂交组合在授粉后05~2h内花粉均可在柱头上大量萌发，在授粉后48h内花粉管均可到达子房与胚珠受精，说明三种甘草种间杂交不存在配子隔离障碍；（3）杂交种子萌发率、发芽指数、平均萌发时间和幼苗成活率与自然结实种子无显著差异，种间杂交种子活力较高，幼苗成活率均为75%以上。以上结果表明3种甘草属植物的同域分布区，在相同传粉途径下，这三种甘草属植物的种间隔离机制不完善，有发生自然杂交并形成杂交区的可能。

关键词: 甘草属植物, 种间杂交, 亲和性, 杂交种子活力

Abstract:

In order to discuss the cross compatibility and hybrid seed vigor of sympatric species among Guralensis, Gglabra and Geurycarpa distributed in Xinjiang, the cross fruiting rate, the pollen germination on stigma, the pollen tube growth in style after cross pollination and germination parameters of hybrid seeds were analyzed. Results showed that there was highly cross compatibility among these three Glycyrrhiza plants and their hybrids seeds were highly vigor. It suggested that the interspecific isolation mechanism of the three Glycyrrhiza plants was weak. Moreover, the natural hybridizations may occur and form natural hybrid zone.

Key words: Glycyrrhiza species, Interspecies cross, Compatibility, Hybrid vigor

中图分类号:

Q 945

谢良碧, 陆嘉惠, 李晓岚, 张宇, 魏涛, 李学禹. 三种甘草属植物的种间杂交亲和性及杂交种子活力[J]. Plant Diversity, 2014, 36(03): 342-348.

XIE Liang-Bi, LU Jia-Hui, LI Xiao-Lan, ZHANG Yu, WEI Tao, LI Xue-Yu. The Cross Compatibility and Hybrid Seed Vigor among Three Glycyrrhiza Species[J]. Plant Diversity, 2014, 36(03): 342-348.

参考文献

陈天华, 徐进, 赖焕林等, 1994. 南京地区黑松与马尾松的自然杂交研究［J］. 南京林业大学学报, 18 (4): 6—12
廖云海, 2011. 两种药用甘草繁育系统的研究［D］. 新疆石河子: 石河子大学
刘育萍, 1994. 晚熟老芒麦与披碱草种间天然远缘杂种的细胞遗传学研究［J］. 内蒙古草业, 1 (2): 60—62
孟雷, 2005. 甘草属(Glycyrrhiza L.) 的系统学研究——兼论与假甘草属(Glycyrrhizopsis Boiss. & Bal.) 的关系［D］. 北京: 中国科学院植物研究所, 11—15
潘家驹, 1992. 作物育种学总论［M］. 北京: 农业出版社
于福来, 2012. 甘草优良种质遴选指标与传粉特性研究［D］. 北京: 北京中医药大学
张富民, 1995. 中国甘草属分类系统与演化［D］. 新疆石河子: 石河子大学
Ashurmetov OA, 1996. Selection of parental pairs for obtaining hybrids in the genera Glycyrrhiza L. and Meristotropis Fisch. et Mey［J］. Genetic Resources Crop Evolution, 43: 167—171
Carrie AE, Benjamin MC, Neil OA et al., 2012. A novel pollen tube growth assay utilizing a transmitting tractablated Nicotiana tabacum style［J］. Sexual Plant Reproduction, 25: 27—37
Du YJ (杜玉娟), Wang YJ (王云瑾), Yang J (杨洁) et al., 2008. Morphological analysis of Hippophae rhamnoides ssp. sinensis × Hippophae neurocarpa ssp. neurocarpa hybrid zone
［J］. Journal of Northwest Normal University (Natural Science) (西北师范大学学报自然科学版), 44 (3): 73—77
Du MF (杜明凤), Chen QF (陈庆富), 2013. Studies on morphology of F1 hybrid and systematic evolution of Epimedium species ［J］. Seed (种子), 32 (8): 17—20
Fang KD, Peng XL, Liu JQ et al., 2011. Direction and extent of organelle DNA introgression between two spruce species in the QinghaiTibetan Plateau［J］. New Phytologist, 192: 1024—1033
Gan T (甘甜), Li QJ (李庆军), 2010. The crossability and hybrid seed vigor among several sympatric Hedychium (Zingiberaceae) species［J］. Acta Botanica Yunnanica (云南植物研究), 32 (3): 230—238
Karapanos IC, Akoumianakis KA, Olympios CM et al., 2010. Tomato pollen respiration in relation to in vitro germination and pollen tube growth under favorable and stressinducing temperatures［J］. Sexual Plant Reproduction, 23: 219—224
Kelly EZ, Meral TO, Jeffrey FH, 2010. Temperature stress and plant sexual reproduction: uncovering the weakest links［J］. Journal of Experimental Botany, 61: 1959—1968
Li XF (李秀凤), 2010. Study on characteristies of flowering and pollination on biology of Glycyrriza uralensis Fisch［D］. Hebei: Agricultural University of Hebei
Li XY (李学禹), 1993. A study of the system and new taxa of genus Glycyrrhiza L［J］. Bulletin of Botanical Research (植物研究), 13: 13—43
Li Y (李亚), Zhang YH (张莹花), Wang JH (王继和) et al., 2007. The influence of different salt stress on seed germination of Haloxylon ammodendron［J］. Chinese Agricultural Science Bulletin (中国农学通报), 23 (9): 293—297
Liu YL (刘亚令), Li ZZ (李作洲), Jiang ZW (姜正旺) et al., 2008. Genetic structure and hybridization introgression in natural populations of two closely related Actinidia species, Achinensis and Adeliciosa［J］. Journal of Plant Ecology (植物生态学报), 32 (3): 704—718
Oliver SN, Van Dongen JT, Alfred SC et al., 2005. Coldinduced repression of the rice antherspecific cell wall invertase gene OSINV4 is correlated with sucrose accumulation and pollen sterility［J］. Plant, Cell and Environment, 28: 1534—1551
Srinivasan A, Saxena NP, Johansen C, 1999. Cold tolerance during early reproductive growth of chickpea (Cicer arietinum L.): genetic variation in gamete development and function［J］. Field Crops Research, 60: 209—222
Tian RW (田润炜), Lu JH ( 陆嘉惠), Xie LB (谢良碧) et al., 2012. Effect of flowering mode and pollination on reproductive success and the relationship between Glycyrrhiza glabra L. and Glycyrrhiza uralensis Fisch［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 32 (10): 2004—2008
van Tuyl JM, Straathof TP, Bino RJ et al., 1998. Effect of three pollinaton methods on embryo development and seedset in intra and interspecific cross between seven Lilium species ［J］. Sexual Plant Reproduction, 1: 119—123
Xu BS (徐炳声), Gu DX (顾德兴), 1986. The role of hybridization in plant evolution and methods of recognizing and naming hybrids［J］. Journal of Wuhan Botanical Research (武汉植物学研究), 4 (4): 385—397
Zhang JL (张敬丽), Zhang CQ (张长芹), Wu ZK (吴之坤) et al., 2007.The potential roles of interspecific pollination in natural hybridization of Rhododendron species in Yunnan, China ［J］. Biodiversity Science (生物多样性), 15 (6): 658—665
Zhang XL (张新玲), Li XY (李学禹), Wei LJ (魏凌基) et al., 1998. The interspecific hybridization of Glycyrrhiza in Xinjiang［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 18 (1): 132—136
Zhang YN (张玉娜), 2009. Study of reproductive isolation to natural hybrid zone of Hippophae (Elaeagnaceae) ［D］. Gansu: Northwest Normal University
Zhao HB (赵宏波), Zhou LH (周莉花), Hao RM (郝日明), 2010. Interspecific cross compatibility between Chimonanthus praecox, Csalicifolius and Czhejiangensis［J］. Journal of Beijing Forestry University (北京林业大学学报), 32 (2): 157—159

[1]	姚恒1, 郑昀晔1, 索文龙2, 马文广1. 烟草种间杂交亲和性[J]. Plant Diversity, 2012, 34(5): 459-465.
[2]	万金鹏1、2 , 朱兴福1 , 李庆军1. 雌先熟植物茄参（茄科）的繁育系统[J]. Plant Diversity, 2011, 33(5): 565-570.
[3]	甘甜1,2，李庆军1 . 几种同域分布姜花属植物的种间杂交亲和性[J]. Plant Diversity, 2010, 32(03): 230-238.
[4]	王赟1 , 2 , 罗建3 , 许建初1 . 铃铛子和赛莨菪( 茄科) 的传粉机制比较研[J]. Plant Diversity, 2009, 31(03): 211-218.
[5]	龚洵　潘跃芝　杨志云　 . 木兰科植物的杂交亲和性[J]. Plant Diversity, 2001, 23(03): 1-3.
[6]	李映红郭仲琛王伏雄. 向日葵的幼胚培养[J]. Plant Diversity, 1988, 10(01): 1-3.
[7]	梁汉兴张赞英张香兰. 云南野山茶与金花茶杂交有性过程的观[J]. Plant Diversity, 1986, 8(02): 1-3.
[8]	徐炳声. 植物分类学中的繁殖生物学问题[J]. Plant Diversity, 1985, 7(04): 1-3.

三种甘草属植物的种间杂交亲和性及杂交种子活力

The Cross Compatibility and Hybrid Seed Vigor among Three Glycyrrhiza Species

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价