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Plant Diversity ›› 2015, Vol. 37 ›› Issue (01): 105-112.DOI: 10.7677/ynzwyj201514045

• 研究论文 • 上一篇    

滇龙胆与青叶胆环烯醚萜类物质计量特征分析

 沈涛1, 黄衡宇2, 张霁3, 赵艳丽3, 金航3, 王元忠3   

  1. 1 玉溪师范学院资源环境学院,云南 玉溪653100;2 云南中医学院中药学院,昆明650500;
    3 云南省农业科学院药用植物研究所,昆明650200
  • 收稿日期:2014-03-16 出版日期:2015-01-25 发布日期:2014-07-25
  • 基金资助:

    国家自然科学基金 (81260608, 31260077);云南省自然科学基金 (2013FD050, 2013FZ150, 2013FD066)

Chemometrics Characterization of Iridoids in Gentiana rigescens and Swertia mileensis

 SHEN  Chao-1, HUANG  Heng-Yu-2, ZHANG  Ji-3, ZHAO  Yan-Li-3, JIN  Hang-3, WANG  Yuan-Zhong-3   

  1. 1 College of Resource and Environment, Yuxi Normal University, Yuxi, Yunnan 653100, China; 2 College of Traditional Chinese
    Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; 3 Institute of
    Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
  • Received:2014-03-16 Online:2015-01-25 Published:2014-07-25
  • Supported by:

    国家自然科学基金 (81260608, 31260077);云南省自然科学基金 (2013FD050, 2013FZ150, 2013FD066)

摘要:

龙胆科龙胆属滇龙胆 (Gentiana rigescens) 与獐牙菜属青叶胆 (Swertia mileensis)为我国特有种。采用高效液相色谱法测定其中马钱苷酸、獐牙菜苦苷、龙胆苦苷和当药苷含量,结合多变量分析研究环烯醚萜类成分含量及其构成比例在龙胆科植物种内和种间的差异。研究结果显示,4种环烯醚萜类成分的计量特征呈现种间差异,依据环烯醚萜类成分含量及其构成比例,所有滇龙胆样品可分为4类。马钱苷酸含量与纬度呈极显著负相关 (R=-0348,P<005),与海拔呈极显著正相关 (R=0307,P<001);獐牙菜苦苷含量与经度呈极显著负相关 (R=-0592,P<001),高海拔有利于植株中当药苷构成比例的增加 (R=0245,P<005);地理因子对植株中龙胆苦苷含量变化影响不显著 (P>005)。

关键词: 滇龙胆, 青叶胆, 环烯醚萜, 变化, 多变量分析

Abstract:

Gentiana rigescens and Swertia mileensis belonged to Gentianaceae are endemic plants in China. Four iridoids (loganic acid, swertiamarin, gentiopicroside and sweroside) in Grigescens and Smileensis were determined by HPLCDAD. Their chemometrics characterizations were analyzed with multiple statistical analyses. The results showed that there were differences of chemometrics characterization of the iridoids between the two species. All the samples of Grigescens could be classified into four groups based on contents and percentage contents of the four iridoids. Content of loganic acid had significant negative correlation with longitude (R=-0348,P<005) and had significant positive correlation with altitude (R=0307, P<001). Longitude had significant negative correlation with content of swertiamarin (R=-0592, P<001) and had significant positive correlation with percentage content of sweroside (R=0245, P<005). However, content of gentiopicroside hadn’t correlation with geographical factors (P>005) in the study.

Key words: Gentiana rigescens, Swertia mileensis, Iridoids, Variation, Multiple statistical analyses

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