不同居群紫花针茅响应干旱胁迫的生理和分子差异分析

doi:10.7677/ynzwyj201514126

摘要/Abstract

摘要：

紫花针茅 (Stipa purpurea) 在青藏高原沿广阔的降水梯度分布。长期的适应过程中，不同地域的紫花针茅可能对干旱具有不同的响应方式。本研究以两个居群的紫花针茅为材料，对干旱胁迫14天和随后的复水过程中各自的生理和分子水平的变化差异进行研究。结果发现两个居群的紫花针茅在植株死亡率、叶片相对含水量、叶绿素荧光、活性氧积累、脯氨酸含量、抗氧化酶活性和抗旱相关基因表达量的变化等方面都有明显差异；且复水后各项指标的恢复水平也不相同。分析表明来自降水较少的普兰地区的紫花针茅的后代与来自降水较多的措勤地区的相比表现出更强的耐旱性，说明不同居群的紫花针茅对干旱的响应差异可能是遗传性。本研究有助于认识紫花针茅在环境中的适应和进化，以及对气候变化的响应。

关键词: 紫花针茅, 青藏高原, 干旱, 适应和进化, 气候变化

Abstract:

Stipa purpurea Griseb, the dominant species of alpine steppe, is widely distributed across the large precipitation gradient on the Tibetan Plateau. It is possible that because of local adaptation, Spurpurea populations from different habitats along this precipitation gradient respond differently to drought, which may affect their responses to climate change. To explore the problem, in the present study, we investigated the physiological and molecular response of Spurpurea seedlings from two different populations (Pulan & Cuoqin) to 14d drought stress and subsequent recovery. The results showed that the relative water content, chlorophyll fluorescence, content of osmoticant proline and malondialdehyde (indicator of oxidative stress), accumulation of reactive oxygen species, antioxidant enzyme activities and the expression of droughtrelated genes all changed under drought treatment and went back to the control levels in the subsequent recovery in plants from Pulan. However, these patterns were quite different in plants from Cuoqin, in which these traits changed by inconsistent degrees and did not return to pretreatment levels after rewatering. The results demonstrated that the plants from Pulan had greater resistance to drought stress compared with those from Cuoqin, which had a larger mortality rate ultimately. Combating the differences of offspring in response to drought and the habitat distribution of parents, we considered that genetic basis has been obtained in response to precipitation difference among Spurpurea populations. The results help to understand the adaptation and evolution of Spurpurea to the special environment and the effect of climate change to this botanical system.

Key words: Stipa purpurea, Tibetan Plateau, Drought, Adaptation and evolution, Climate change

中图分类号:

Q 945

李雄1、2、3, 杨时海3、4, 杨云强1、2, 尹欣1、2、3, 孙旭东1、2, 杨永平1、2. 不同居群紫花针茅响应干旱胁迫的生理和分子差异分析[J]. Plant Diversity, 2015, 37(4): 439-452.

LI Xiong-, YANG Shi-Hai-, YANG Yun-Qiang-, YIN Xin-, SUN Xu-Dong-, YANG Yong-Ping. Comparative Physiological and Molecular Analyses of Intraspecific Differences of Stipa purpurea (Poaceae) Response to Drought[J]. Plant Diversity, 2015, 37(4): 439-452.

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